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Invitrogen
POT1 Polyclonal Antibody
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FIGURE: 1 / 3
POT1 Antibody (PA5-75366) in WB
Western blot analysis of POT1 in Lane 1: 786-O whole cell lysate (40 µg), Lane 2: PC3 whole cell lysate (40 µg), Lane 3: the Testis tissue lysate of mouse (40 µg), Lane 4: the Testis tissue lysate of rat (40 µg). Samples were incubated with POT1 polyclonal antibody (Product # PA5-75366) at a dilution of 1:500.
Product Details
PA5-75366
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide corresponding to amino acids 269-315 of Human POT1.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Target Information
POT1 is a component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. It is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Pot1 also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. It binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Its activity is TERT dependent but it does not increase TERT activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DKFZp586D211; hPot1; mPot1; POT 1; POT-1; POT1-like telomere end-binding protein; protection of telomeres 1 homolog; protection of telomeres 1A; Protection of telomeres protein 1; telomere binding protein; hPot1; unnamed protein product
Gene Aliases: 1500031H18Rik; AI851169; CMM10; CRMCC3; GLM9; HPOT1; PFBMFT8; POT1; Pot1a; TPDS3
UniProt ID: (Human) Q9NUX5, (Mouse) Q91WC1
Entrez Gene ID: (Human) 25913, (Rat) 500054, (Mouse) 101185
DNA binding
protein binding
telomerase inhibitor activity
DEAD/H-box RNA helicase binding
telomeric DNA binding
single-stranded telomeric DNA binding
telomeric D-loop binding
telomeric G-quadruplex DNA binding
G-rich strand telomeric DNA binding
8-hydroxy-2'-deoxyguanosine DNA binding
G-rich single-stranded DNA binding
DNA metabolism protein
telomere maintenance
telomere maintenance via telomerase
telomere capping
telomere assembly
positive regulation of telomere maintenance
regulation of telomere maintenance via telomerase
negative regulation of telomere maintenance via telomerase
positive regulation of telomere maintenance via telomerase
positive regulation of DNA strand elongation
telomeric D-loop disassembly
establishment of protein localization to telomere
positive regulation of telomeric D-loop disassembly
regulation of double-strand break repair via nonhomologous end joining
DNA duplex unwinding
positive regulation of helicase activity
chromosome organization
positive regulation of telomerase activity
negative regulation of telomerase activity
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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