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PP2A Antibody (A300-732A) in WB
Detection of human and mouse PP2A by western blot. Samples: Whole cell lysate (50 µg) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-PP2A antibody A300-732A (lot A300-732A-2) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A300-732A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 260 and the C-terminus (residue 309) of Human Protein Phosphatase 2A (PPP2CB, Protein Phosphatase 2, Catalytic Subunit, Beta isoform).
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat, D. melanogaster, Chicken, Bovine, Rabbit and Pig
Target Information
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes a beta isoform of the catalytic subunit. Two transcript variants encoding the same protein have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: PP2A beta; PP2A-beta; PPP2bc; protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform; protein phosphatase 2, catalytic subunit, beta isoform; protein phosphatase 2, catalytic subunit, beta isozyme; protein phosphatase 2A catalytic subunit, beta isoform; protein phosphatase 2a, catalytic subunit, beta isoform; protein phosphatase type 2A catalytic subunit; serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform; Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform; testicular tissue protein Li 146
Gene Aliases: AI115466; D8Ertd766e; PP2Abeta; PP2Ac; PP2CB; PPP2CB
UniProt ID: (Human) P62714, (Mouse) P62715
Entrez Gene ID: (Human) 5516, (Mouse) 19053
protein dephosphorylation
apoptotic mitochondrial changes
regulation of gene expression
response to endoplasmic reticulum stress
response to hydrogen peroxide
proteasome-mediated ubiquitin-dependent protein catabolic process
response to antibiotic
negative regulation of Ras protein signal transduction
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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