|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 129 and 378 of Human PPAR delta|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Contains||0.025% Proclin 300|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:500-1:3000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 1 publications below|
PA5-29678 targets PPAR delta in IHC (P) and WB applications and shows reactivity with Human and mouse samples.
The PA5-29678 immunogen is recombinant fragment corresponding to a region within amino acids 129 and 378 of Human PPAR delta.
This gene encodes a member of the peroxisome proliferator-activated receptor (PPAR) family. PPARs are nuclear hormone receptors that bind peroxisome proliferators and control the size and number of peroxisomes produced by cells. PPARs mediate a variety of biological processes, and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer. This protein is a potent inhibitor of ligand-induced transcription activity of PPAR alpha and PPAR gamma. It may function as an integrator of transcription repression and nuclear receptor signaling. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein related to APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, lipid metabolism, and epidermal cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Deubiquitinase DUBA is a post-translational brake on interleukin-17 production in T cells.
PA5-29678 was used in western blot to identify DUBA as a suppressor of IL-17 production in T cells.
|Rutz S,Kayagaki N,Phung QT,Eidenschenk C,Noubade R,Wang X,Lesch J,Lu R,Newton K,Huang OW,Cochran AG,Vasser M,Fauber BP,DeVoss J,Webster J,Diehl L,Modrusan Z,Kirkpatrick DS,Lill JR,Ouyang W,Dixit VM||Nature (518:417)||2015|