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Invitrogen
PPCS Polyclonal Antibody
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FIGURE: 1 / 8
PPCS Antibody (PA5-95630) in ICC/IF
Immunofluorescence analysis of PPCS in A431 cell. Antigen retrieval was performed on the tissue using IHC enzyme antigen retrieval reagent and blocked with 10% goat serum. Samples were incubated with PPCS polyclonal antibody (Product # PA5-95630) at a 2 µg/1x10^6 cells dilution, followed by 488 conjugated goat anti-rabbit IgG using a 1:100 dilution.
Product Details
PA5-95630
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence of human PPCS (VEIEEKIVDNLQSRHTAFIGDRN).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human placenta tissue, human PC-3 whole cell. IHC: human intestinal cancer tissue, mouse small intestine tissue, rat small intestine tissue. ICC/IF: A549 cell, A431 cell. Flow: A431 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCS (EC 6.3.2.5), one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Phosphopantothenate--cysteine ligase; Phosphopantothenoylcysteine synthetase; PPC synthetase
Gene Aliases: 6330579B17Rik; AI413471; AI790434; COAB; PPCS; RGD1311399
UniProt ID: (Human) Q5VVM0, (Mouse) Q8VDG5
Entrez Gene ID: (Human) 79717, (Mouse) 106564, (Rat) 298490
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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