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PPHLN1 Antibody (PA5-120574) in WB
Western blot analysis of PPHLN1 in extracts of various cell lines. Samples were incubated with PPHLN1 Polyclonal antibody (Product # PA5-120574) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-120574
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 195-255 of human PPHLN1 (NP_958846.1).
Conjugate
Form
Liquid
Concentration
1.26 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914146
Product Specific Information
Positive test controls include: U-87MG, THP-1, Mouse testis, Rat testis, Rat spleen, Rat lung.
Immunogen sequence: KSDESNLPEI SEYEAGSTAP LFTDQPEEPE SNTTHGIELF EDSQLTTRSK AIASKTKEIE Q
Target Information
The protein encoded by this gene is one of the several proteins that become sequentially incorporated into the cornified cell envelope during the terminal differentiation of keratinocyte at the outer layers of epidermis. This protein interacts with periplakin, which is known as a precursor of the cornified cell envelope. The cellular localization pattern and insolubility of this protein suggest that it may play a role in epithelial differentiation and contribute to epidermal integrity and barrier formation. Multiple alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC7 expression repressor; CR; Gastric cancer antigen Ga50; MGC48786; Periphilin-1
Gene Aliases: CR; HSPC206; HSPC232; PPHLN1
UniProt ID: (Human) Q8NEY8, (Mouse) Q8K2H1
Entrez Gene ID: (Human) 51535, (Mouse) 223828, (Rat) 366975
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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