Invitrogen

PPP1R8 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (30) PPP1R8 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Tech Support

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PPP1R8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent staining of PPP1R8 in human cell line A-431 using a PPP1R8 Polyclonal Antibody (Product # PA5-55601) shows localization to nuclear speckles.

PPP1R8 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of PPP1R8 in human thyroid gland using a PPP1R8 Polyclonal Antibody (Product # PA5-55601) shows strong nuclear positivity in glandular cells.

PPP1R8 Antibody in Western Blot (WB) — Western blot analysis of PPP1R8 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using a PPP1R8 Polyclonal Antibody (Product # PA5-55601).

PPP1R8 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U2OS cells were transfected with, control siRNA, PPP1R8 siRNA probe #1, and PPP1R8 siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-PPP1R8 Polyclonal Antibody (Product # PA5-55601) used at a dilution of 0.4 µg/mL. {KD}

Product Details

PA5-55601

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human PPP1R8 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2645880

Product Specific Information

Immunogen sequence: ISTLTIEEGN LDIQRPKRKR KNSRVTFSED DEIINPEDVD PSVGRFRNMV QTAVVPVKKK RVEGPGSLGL EESGSRRMQN FAFSGGLY

Highest antigen sequence identity to the following orthologs: Mouse - 98%, Rat - 97%.

Target Information

This gene, through alternative splicing, encodes three different isoforms. Two of the protein isoforms encoded by this gene are specific inhibitors of type 1 serine/threonine protein phosphatases and can bind but not cleave RNA. The third protein isoform lacks the phosphatase inhibitory function but is a single-strand endoribonuclease comparable to RNase E of E. coli. This isoform requires magnesium for its function and cleaves specific sites in A+U-rich regions of RNA.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Activator of RNA decay; ARD-1; NIPP-1; Nuclear inhibitor of protein phosphatase 1; nuclear inhibitor of protein phosphatase-1 alpha; nuclear inhibitor of protein phosphatase-1 beta; nuclear subunit of PP-1; Protein phosphatase 1 regulatory inhibitor subunit 8; protein phosphatase 1, regulatory (inhibitor) subunit 8; protein phosphatase 1, regulatory subunit 8; RNase E

Gene Aliases: 6330548N22Rik; ARD-1; ARD1; AU044684; NIPP-1; NIPP1; PPP1R8; PRO2047

UniProt ID: (Human) Q6PKF6, (Mouse) Q8R3G1

Entrez Gene ID: (Human) 5511, (Rat) 313030, (Mouse) 100336

Molecular Function: RNA splicing factor