Proteintech

PPP2R1A Polyclonal Antibody

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PPP2R1A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human ovary tumor using 15882-1-AP (PPP2R1A antibody) at dilution of 1:50 (under 40x lens).

PPP2R1A Antibody in Western Blot (WB) — Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15882-1-AP (PPP2R1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

PPP2R1A Antibody in Immunoprecipitation (IP) — IP result of anti-PPP2R1A (IP:15882-1-AP, 4ug; Detection:15882-1-AP 1:300) with HepG2 cells lysate 3200ug.

Product Details

15882-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 5 publications 5 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Pig

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PPP2R1A Fusion Protein Ag8661 (239-589 aa encoded by BC001537)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.16 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: DLEALVMPT LRQAAEDKSW RVRYMVADKF TELQKAVGPE ITKTDLVPAF QNLMKDCEAE VRAAASHKVK EFCENLSADC RENVIMSQIL PCIKELVSDA NQHVKSALAS VIMGLSPILG KDNTIEHLLP LFLAQLKDEC PEVRLNIISN LDCVNEVIGI RQLSQSLLPA IVELAEDAKW RVRLAIIEYM PLLAGQLGVE FFDEKLNSLC MAWLVDHVYA IREAATSNLK KLVEKFGKEW AHATIIPKVL AMSGDPNYLH RMTTLFCINV LSEVCGQDIT TKHMLPTVLR MAGDPVANVR FNVAKSLQKI GPILDNSTLQ SEVKPILEKL TQDQDVDVKY FAQEALTVLS LA (239-589 aa encoded by BC001537)

Target Information

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Mon May 08 00:00:00 EDT 2023

Fam72a functions as a cell-cycle-controlled gene during proliferation and antagonizes apoptosis through reprogramming PP2A substrates.

Developmental cell

Fu Y,Jia X,Yuan J,Yang Y,Zhang T,Yu Q,Zhou J,Wang T

Mon Mar 13 00:00:00 EDT 2023

TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells.

Microbiology spectrum

Jiang C,Ma N,Cao H,Zeng W,Ren J,Hu Y,Zhou J,Zhang M,Li C,Lang Y,Li W,He Q

Wed Oct 26 00:00:00 EDT 2022

TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells.

Microbiology spectrum

Jiang C,Ma N,Cao H,Zeng W,Ren J,Hu Y,Zhou J,Zhang M,Li C,Lang Y,Li W,He Q

Wed Oct 26 00:00:00 EDT 2022

TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells.

Microbiology spectrum

Jiang C,Ma N,Cao H,Zeng W,Ren J,Hu Y,Zhou J,Zhang M,Li C,Lang Y,Li W,He Q

Wed Oct 26 00:00:00 EDT 2022

TurboID Screening of the OmpP2 Protein Reveals Host Proteins Involved in Recognition and Phagocytosis of Glaesserella parasuis by iPAM Cells.

Microbiology spectrum

Jiang C,Ma N,Cao H,Zeng W,Ren J,Hu Y,Zhou J,Zhang M,Li C,Lang Y,Li W,He Q

Wed Oct 26 00:00:00 EDT 2022

SPT5 stabilizes RNA polymerase II, orchestrates transcription cycles, and maintains the enhancer landscape.

Molecular cell

Hu S,Peng L,Xu C,Wang Z,Song A,Chen FX

Thu Nov 04 00:00:00 EDT 2021

Identification of Integrator-PP2A complex (INTAC), an RNA polymerase II phosphatase.

Science (New York, N.Y.)

Zheng H,Qi Y,Hu S,Cao X,Xu C,Yin Z,Chen X,Li Y,Liu W,Li J,Wang J,Wei G,Liang K,Chen FX,Xu Y

Fri Nov 27 00:00:00 EST 2020

BRG1 Activates PR65A Transcription to Regulate NO Bioavailability in Vascular Endothelial Cells.

Frontiers in cell and developmental biology

Chen B,Zhao Q,Xu T,Yu L,Zhuo L,Yang Y,Xu Y

Mon Sep 28 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: alpha isoform of regulatory subunit A, protein phosphatase 2; Medium tumor antigen-associated 61 kDa protein; MGC786; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; PP2Ac; PP2Calpha; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform; protein phosphatase 2 regulatory subunit A, alpha; protein phosphatase 2, regulatory subunit A, alpha; protein phosphatase PP2A; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform; serine/threonine protein phosphatase A subunit type 2A; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; testicular secretory protein Li 1

Gene Aliases: 6330556D22Rik; MRD36; PP2A; PP2A-Aalpha; PP2AAALPHA; PPP2R1A; PR65; PR65A

UniProt ID: (Human) P30153, (Mouse) Q76MZ3

Entrez Gene ID: (Human) 5518, (Mouse) 51792, (Rat) 117281

Molecular Function: phosphatase modulator