Invitrogen

PPP2R1A Polyclonal Antibody

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Datasheet

Protocols

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PPP2R1A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PPP2R1A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PPP2R1A Polyclonal Antibody (Product # PA5-27643) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing a predominant nuclear localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PPP2R1A Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of PPP2R1A was performed in paraffin-embedded mouse kidney tissue using PPP2R1A Polyclonal Antibody (Product # PA5-27643) at a dilution of 1:2500.

PPP2R1A Antibody in Western Blot (WB) — Western blot was performed using Anti-PPP2R1A Polyclonal Antibody(Product # PA5-27643) and a 60kDa band corresponding to PPP2R1A was observed across all the tested cell lines and tissues. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MDA-MB-231 (Lane 2), MCF7 (Lane 3), Jurkat (Lane 4), NIH/3T3 (Lane 5), L6 (Lane 6), Mouse Brain (Lane 7), Rat Brain (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Product Details

PA5-27643

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:10,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:2500	-
Immunocytochemistry (ICC/IF)	1:100-1:1,000	-
Immunoprecipitation (IP)	Assay-dependent	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 308 and 589 of Human PPP2R1A View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.12 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% ProClin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2545119

Product Specific Information

Recommended positive controls: HeLa, NIH-3T3, JC, BCL-1.

Predicted reactivity: Mouse (100%), Rat (100%), Zebrafish (88%), Xenopus laevis (95%), Pig (100%), Rhesus Monkey (100%), Bovine (99%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha isoform of regulatory subunit A, protein phosphatase 2; Medium tumor antigen-associated 61 kDa protein; MGC786; PP2A subunit A isoform PR65-alpha; PP2A subunit A isoform R1-alpha; PP2Ac; PP2Calpha; protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform; protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform; protein phosphatase 2 regulatory subunit A, alpha; protein phosphatase 2, regulatory subunit A, alpha; protein phosphatase PP2A; serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit A, alpha isoform; serine/threonine protein phosphatase A subunit type 2A; Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform; testicular secretory protein Li 1

Gene Aliases: 6330556D22Rik; MRD36; PP2A; PP2A-Aalpha; PP2AAALPHA; PPP2R1A; PR65; PR65A

UniProt ID: (Human) P30153, (Mouse) Q76MZ3

Entrez Gene ID: (Human) 5518, (Mouse) 51792, (Rat) 117281

Molecular Function: phosphatase modulator