Invitrogen

PPP2R2A Recombinant Polyclonal Antibody (1HCLC)

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Datasheet

Protocols

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PPP2R2A Antibody in Immunocytochemistry (ICC/IF) — For immunofluorescence analysis, HeLA cells were fixed and permeabilized for detection of endogenous PP2AB using Anti- PP2AB Recombinant Rabbit Polyclonal Antibody (Product # 710874, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of PP2AB protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of PP2AB. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PPP2R2A Antibody in Western Blot (WB) — Western blot analysis was performed on Membrane enriched extracts (30 µg lysate) of C2C12 (Lane 1), NIH/3T3 (Lane 2) and tissue extracts of Mouse Testis (Lane 3), Mouse Brain (Lane 4) and Rat Brain (Lane 5). The blots were probed with Anti-PP2A B Recombinant Rabbit Polyclonal Antibody (Product # 710874, 2.5 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 36 kDa band corresponding to PP2A B was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Product Details

710874

Applications	Tested Dilution	Publications
Western Blot (WB)	2.5 µg/mL	-
Immunocytochemistry (ICC/IF)	5 µg/mL	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Recombinant Polyclonal
Type	Antibody
Clone	1HCLC
Immunogen	Peptides corresponding to human PP2AB [1) aa133-aa152 2) aa55-aa70] View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Protein A
Storage buffer	PBS, pH 7.4
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2716896

Product Specific Information

This antibody predicted to react with Monkey, Mouse, Pig

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

Target Information

The product of this gene belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B55 subfamily. Alternatively spliced transcript variants have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: PP2A subunit B isoform alpha; PP2A subunit B isoform B55-alpha; PP2A subunit B isoform BRA; PP2A subunit B isoform PR55-alpha; PP2A subunit B isoform R2-alpha; PP2A, subunit B, B-alpha isoform; PP2A, subunit B, B55-alpha isoform; PP2A, subunit B, BRA isoform; PP2A, subunit B, PR55-alpha isoform; PP2A, subunit B, R2-alpha isoform; protein phosphatase 2 (formerly 2A), regulatory subunit B (PR 52), alpha isoform; protein phosphatase 2 (formerly 2A), regulatory subunit B (PR52), alpha isoform; protein phosphatase 2 (formerly 2A), regulatory subunit B, alpha isoform; protein phosphatase 2 regulatory subunit B, alpha; protein phosphatase 2, regulatory subunit B, alpha; serine/threonine protein phosphatase 2A, 55 KDA regulatory subunit B, alpha isoform; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform; testicular tissue protein Li 156

Gene Aliases: 2410004D02Rik; B55A; B55ALPHA; mKIAA1541; PPP2R2A; PR52A; PR55A

UniProt ID: (Human) P63151, (Mouse) Q6P1F6, (Rat) P36876

Entrez Gene ID: (Human) 5520, (Mouse) 71978, (Rat) 117104

Molecular Function: protein phosphatase