Invitrogen

PPP2R2D Polyclonal Antibody

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PPP2R2D Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (Paraffin) analysis of PPP2R2D was performed in paraffin-embedded mouse duodenum tissue using PPP2R2D Polyclonal Antibody (Product # PA5-30763) at a dilution of 1:500.

PPP2R2D Antibody in Western Blot (WB) — Western blot analysis of PPP2R2D was performed by separating 50 µg of various tissue extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a PPP2R2D Polyclonal Antibody (Product # PA5-30763) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

PPP2R2D Antibody in Western Blot, Immunoprecipitation (WB, IP) — Figure 3. CDC25B interacts directly with the PP2A-C subunit. ( A ) Top: GST-CDC25B was used to pull down purified His-PP2A-A, His-B56delta, His-PP2A-C, His-PP2A-A + B56delta, His-PP2A-A + PP2A-C, His-B56delta + PP2A-C or His-PP2A-A + B56delta + PP2A-C. Bottom: GST immunoblot of SDS-PAGE gel demonstrating the amount of CDC25B used for pull-down experiments. ( B and C ) The CDC25B-B56delta interaction in Neg or PP2A-C KD cells. BT549 and HS578T cells were transfected with Neg or siPP2A-C. Forty-eight hours later, cell lysates were subjected to IP with control IgG or anti-CDC25B antibody examining the CDC25B-B56delta interaction. Reciprocal IP with B56delta antibody was also performed to examine the CDC25B-B56delta interaction. The immunoprecipitates were blotted with the indicated antibodies. ( D ) B56delta, not B55delta, interacts with CDC25B. BT549 cells were transfected with siB56delta. Forty-eight hours later, cell lysates were subjected to IP with control IgG or anti-CDC25B antibody examining the CDC25B-B55delta interaction. Reciprocal IP with B55delta antibody was also performed to exam the CDC25B-B55delta interaction. The immunoprecipitates were blotted with the indicated antibodies. Western blot analysis demonstrates the protein level of B55delta in Neg or B56delta KD cells. Data information: all data presented are a representation of N = 3 independent experiments.

Product Details

PA5-30763

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:10,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-
Immunoprecipitation (IP)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 91 and 380 of Human PPP2R2D View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 20% glycerol
Contains	0.025% Proclin 300
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2548237

Product Specific Information

Recommended positive controls: IMR32, mouse brain, rat brain.

Predicted reactivity: Mouse (99%), Rat (99%), Zebrafish (95%), Xenopus laevis (95%), Chicken (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PPP2R2D is the B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CDC25B partners with PP2A to induce AMPK activation and tumor suppression in triple negative breast cancer.

NAR cancer

Cairns J,Ly RC,Niu N,Kalari KR,Carlson EE,Wang L

PA5-30763 was used in Western Blotting to demonstrate that in addition to its role in cell cycle regulation, CDC25B functions as a regulator of protein phosphatase 2A (PP2A), a major cellular Ser/Thr phosphatase, through its direct interaction with PP2A catalytic subunit.

Tue Dec 01 00:00:00 EST 2020

CDC25B partners with PP2A to induce AMPK activation and tumor suppression in triple negative breast cancer.

NAR cancer

Cairns J,Ly RC,Niu N,Kalari KR,Carlson EE,Wang L

Tue Dec 01 00:00:00 EST 2020

CDC25B partners with PP2A to induce AMPK activation and tumor suppression in triple negative breast cancer.

NAR cancer

Cairns J,Ly RC,Niu N,Kalari KR,Carlson EE,Wang L

Tue Dec 01 00:00:00 EST 2020

Bioinformatics

Protein Aliases: PP2A subunit B isoform B55-delta; PP2A subunit B isoform delta; PP2A subunit B isoform PR55-delta; PP2A subunit B isoform R2-delta; PP2A, subunit B, B-delta isoform; PP2A, subunit B, B55-delta isoform; PP2A, subunit B, PR55-delta isoform; PP2A, subunit B, R2-delta isoform; protein phosphatase 2 regulatory subunit B, delta; protein phosphatase 2, regulatory subunit B, delta isoform; protein phosphatase 2A B regulatory subunit delta isoform; protein phosphatase 2A, regulatory subunit B, delta isoform; Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B delta isoform

Gene Aliases: 1300017E19Rik; D7Ertd753e; KIAA1541; MDS026; PPP2R2D

UniProt ID: (Human) Q66LE6, (Rat) P56932, (Mouse) Q925E7

Entrez Gene ID: (Human) 55844, (Rat) 246255, (Mouse) 52432

Molecular Function: protein phosphatase