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Invitrogen
PPP2R3B Polyclonal Antibody
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FIGURE: 1 / 2
PPP2R3B Antibody (PA5-97082) in WB
Western blot analysis of PPP2R3B in extracts of various cell lines. Samples were incubated with PPP2R3B Polyclonal antibody (Product # PA5-97082) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
Product Details
PA5-97082
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 356-575 of human PPP2R3B (NP_0373712)
Conjugate
Form
Liquid
Concentration
1.03 mg/mL
Amount
103 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: FSGAVTRGRK VQKEGKISYA DFVWFLISEE DKKTPTSIEY WFRCMDLDGD GALSMFELEY FYEEQCRRLD SMAIEALPFQ DCLCQMLDLV KPRTEGKITL QDLKRCKLAN VFFDTFFNIE KYLDHEQKEQ ISLLRDGDSG GPELSDWEKY AAEEYDILVA EETAGEPWED GFEAELSPVE QKLSALRSPL AQRPFFEAPS PLGAVDLYEY ACGDEDLEPL; Positive Samples: HeLa, SW620, BT-474, SKOV3, mouse skeletal muscle; Cellular Location: Nucleus
Target Information
Protein phosphatase 2 is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the beta subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: NY-REN-8; NY-REN-8 antigen; PP2A subunit B isoform PR48; protein phosphatase 2 (formerly 2A), regulatory subunit B'', beta; protein phosphatase 2, regulatory subunit B'', beta; Protein phosphatase 2A 48 kDa regulatory subunit; serine/threonine protein phosphatase 2A, 48kDa regulatory subunit B; Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit beta; similar to protein phosphatase 2A regulatory subunit; unnamed protein product
Gene Aliases: NYREN8; PPP2R3B; PPP2R3L; PPP2R3LY; PR48; PR70
UniProt ID: (Human) Q9Y5P8
Entrez Gene ID: (Human) 28227
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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