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Invitrogen
PRC1 Polyclonal Antibody
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FIGURE: 1 / 8
PRC1 Antibody (PA5-30296) in ICC/IF
Immunofluorescence analysis of PRC1 was performed using 70% confluent log phase HeLa cells (Panel a-d) and HeLa cells arrested in G1 phase using deferoxamine (1mM for 12hrs) (Panel e). The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PRC1 Rabbit Polyclonal Antibody (Product # PA5-30296) at 1:200 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit I... View More
Product Details
PA5-30296
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human PRC1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: Jurkat, Raji, Mouse testis.
Predicted reactivity: Mouse (83%), Rat (81%), Bovine (94%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
This gene encodes a protein that is involved in cytokinesis. The protein is present at high levels during the S and G2/M phases of mitosis but its levels drop dramatically when the cell exits mitosis and enters the G1 phase. It is located in the nucleus during interphase, becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. This protein has been shown to be a substrate of several cyclin-dependent kinases (CDKs). It is necessary for polarizing parallel microtubules and concentrating the factors responsible for contractile ring assembly.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: anaphase spindle elongation 1 homolog; MGC1671; MGC3669; PRC1; protein regulating cytokinesis 1; Protein regulator of cytokinesis 1
Gene Aliases: ASE1; D7Ertd348e; PRC1
UniProt ID: (Human) O43663, (Mouse) Q99K43
Entrez Gene ID: (Human) 9055, (Mouse) 233406
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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