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Immunofluorescent analysis of PRDM14 in breast cancer cells using a PRDM14 polyclonal antibody (Product # PA5-11303). SKBr-3 cells were transfected with pCMV-Tag2A or pCMV-PRDM14 and then labeled with a PRDM14 polyclonal antibody (Product # PA5-11303). The nucleus was stained with DAPI.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PRDM14|
|Purification||Ammonium sulfate precipitation, Size-exclusion - Dialysis|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Similar to acetylation and phosphorylation, histone methylation at the N terminal tail has emerged as an important role in regulating chromatin dynamics and gene activity. Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET domain containing methyltransferase family. Five members have been identified in the arginine methyltransferase family. About 27 are grouped into the SET domain family, and another 17 make up the PR domain family that is related to the SET domain family. PRDM14 is part of a family of PR domain genes that are involved in tumorigenesis. It may function as a transcription factor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
PFM11; PR domain containing 14; PR domain-containing 14; PR domain-containing protein 14