Immunohistochemical analysis of RIZ1 in adenovirus-transduced tumor cells using a PRDM2 polyclonal antibody (Product # PA5-11295). Established HCT116 xenograft tumors at day 2 after infection of AdRIZ1, Adnull, or buffer alone were processed for RIZ1 in situ detection (TUNEL) analysis.
|Tested species reactivity||Human, Mouse, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Purified recombinant GST fusion protein encoding aa 245-573 of rat Riz1.|
|Contains||0.09% sodium azide|
|Storage Conditions||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C|
|Tested Applications||Dilution *|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody also recognizes the isoform beta (Riz2) of PRDM2 (185 kDa).
PRDM2 is a 250-kD nuclear protein containing 8 zinc finger motifs. It contains an RB-binding motif that is related to that of the adenovirus E1A oncoprotein; RIZ also shares an antigenic epitope with the C terminus of E1A. It is expressed in human retinoblastoma cells and at low levels in all other human cell lines examined. Using fluorescence in situ hybridization, the PRDM2 gene has been mapped to 1p36. PRDM2 may be a candidate target of 1p36 alterations that commonly occur in neuroendocrine, breast, liver, colon, skin, and lymphoid tumors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.