Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 8
PRDX6 Antibody (PA5-95236) in ICC/IF
Immunocytochemistry analysis of PRDX6 using anti-PRDX6 antibody (Product # PA5-95236). PRDX6 was detected in a section of PC-3 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 1μg/mL rabbit anti-PRDX6 antibody (Product # PA5-95236) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The section was developed using Strepavidin-Biotin-Complex (SABC) with DA... View More
Product Details
PA5-95236
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Peroxiredoxin 6 recombinant protein (Position: E15-P224).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2807040
Product Specific Information
Human Peroxiredoxin 6 shares 90% and 91% amino acid (aa) sequence identity with mouse and rat Peroxiredoxin 6, respectively.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, human Jurkat whole cell, human MCF-7 whole cell, human 293T whole cell, rat lung tissue, rat liver tissue, mouse lung tissue, mouse liver tissue. IHC: Human Mammary Cancer tissue, human gastric cancer tissue, human Lung cancer tissue, human Ovarian cancer tissue, rat brain tissue. ICC/IF: PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20-30 kilodalton in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 1-Cys peroxiredoxin; 1-Cys PRX; 24 kDa protein; Acidic calcium-independent phospholipase A2; aiPLA2; Antioxidant protein 2; epididymis secretory sperm binding protein Li 128m; Glutathione-dependent peroxiredoxin; Liver 2D page spot 40; LPC acyltransferase 5; Lysophosphatidylcholine acyltransferase 5; MGC46173; MSP23; NKEFA; Non-selenium glutathione peroxidase; NSGPx; PAG; PAGA; PAGB; Peroxiredoxin-6; Prx6; Red blood cells page spot 12; Thiol-specific antioxidant protein
Gene Aliases: 1-Cys; 1-Cys Prx; 1-cysPrx; 9430088D19Rik; AA690119; Aipla2; AOP2; Brp-12; CP-3; GPx; HEL-S-128m; KIAA0106; Ltw-4; Ltw4; Lvtw-4; NSGPx; ORF06; p29; Prdx5; PRDX6; PRX; Tsa
UniProt ID: (Human) P30041, (Mouse) O08709, (Rat) O35244
Entrez Gene ID: (Human) 9588, (Mouse) 11758, (Rat) 94167
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support