Proteintech

PREB Polyclonal Antibody

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PREB Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 10146-2-AP (PREB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PREB Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human prostate cancer tissue slide using 10146-2-AP ( PREB Antibody) at dilution of 1:50 (under 40x lens).

PREB Antibody in Western Blot (WB) — HT-1080 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

PREB Antibody in Immunoprecipitation (IP) — IP result of anti-PREB (IP: 10146-2-AP, 4 µg; Detection: 10146-2-AP 1:500) with SKOV-3 cells lysate 3200 µg.

Product Details

10146-2-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse
Published species	Human, Mouse
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PREB Fusion Protein Ag0202 (13-202 aa encoded by BC002765) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.39 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: PFPLYALQV DPSTGLLIAA GGGGAAKTGI KNGVHFLQLE LINGRLSASL LHSHDTETRA TMNLALAGDI LAAGQDAHCQ LLRFQAHQQQ GNKAEKAGSK EQGPRQRKGA APAEKKCGAE TQHEGLELRV ENLQAVQTDF SSDPLQKVVC FNHDNTLLAT GGTDGYVRVW KVPSLEKVLE FKAHEGEIED L (13-202 aa encoded by BC002765)

Target Information

Prolactin (PRL) expression in the pituitary is limited to specific cells. Pit-1 is a pituitary specific transcription factor that plays an important role in PRL expression, both in mature organism and during development. The PRL promoter contains numerous Pit-1 binding sites and these sites have been implicated in both basal level and kinase-mediated gene expression. The most proximal of these binding sites, termed 1P, has been shown to direct a response to numerous signals, such as cAMP and various G-proteins. A novel protein, termed PREB (prolactin regulatory element binding protein), has been recently identified that regulates PRL gene expression through the 1P site, though it contains no discernable DNA-binding motif. Recent studies suggest that PREB is encoded by a single-copy gene in both mice and humans and exhibits nuclear accumulation in pituitary cells. Evidence suggests that PREB is a novel transcription factor that assists in PRL expression whether alone, or in concert with Pit-1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation.

Biochemical and biophysical research communications

Urai Y,Yamawaki M,Watanabe N,Seki Y,Morimoto T,Tago K,Homma K,Sakagami H,Miyamoto Y,Yamauchi J

Mon Sep 10 00:00:00 EDT 2018

Prolactin regulatory element-binding (PREB) protein regulates hepatic glucose homeostasis.

Biochimica et biophysica acta. Molecular basis of disease

Park JM,Kim MY,Kim TH,Min DK,Yang GE,Ahn YH

Fri Jun 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: Mammalian guanine nucleotide exchange factor mSec12; prolactin regulatory binding-element protein; Prolactin regulatory element-binding protein

Gene Aliases: C85705; PREB; SEC12

UniProt ID: (Human) Q9HCU5, (Mouse) Q9WUQ2

Entrez Gene ID: (Human) 10113, (Mouse) 50907

Molecular Function: guanyl-nucleotide exchange factor