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Search Thermo Fisher Scientific
Applications | Tested Dilution | Publications |
---|---|---|
Western Blot (WB) |
1:500-1:1,000 | View 2 publications 2 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:10-1:100 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
Product Specifications | |
---|---|
Species Reactivity |
Human, Mouse |
Published species |
Human, Mouse |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
PREB Fusion Protein Ag0202 (13-202 aa encoded by BC002765) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.39 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Immunogen sequence: PFPLYALQV DPSTGLLIAA GGGGAAKTGI KNGVHFLQLE LINGRLSASL LHSHDTETRA TMNLALAGDI LAAGQDAHCQ LLRFQAHQQQ GNKAEKAGSK EQGPRQRKGA APAEKKCGAE TQHEGLELRV ENLQAVQTDF SSDPLQKVVC FNHDNTLLAT GGTDGYVRVW KVPSLEKVLE FKAHEGEIED L (13-202 aa encoded by BC002765)
Prolactin (PRL) expression in the pituitary is limited to specific cells. Pit-1 is a pituitary specific transcription factor that plays an important role in PRL expression, both in mature organism and during development. The PRL promoter contains numerous Pit-1 binding sites and these sites have been implicated in both basal level and kinase-mediated gene expression. The most proximal of these binding sites, termed 1P, has been shown to direct a response to numerous signals, such as cAMP and various G-proteins. A novel protein, termed PREB (prolactin regulatory element binding protein), has been recently identified that regulates PRL gene expression through the 1P site, though it contains no discernable DNA-binding motif. Recent studies suggest that PREB is encoded by a single-copy gene in both mice and humans and exhibits nuclear accumulation in pituitary cells. Evidence suggests that PREB is a novel transcription factor that assists in PRL expression whether alone, or in concert with Pit-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: Mammalian guanine nucleotide exchange factor mSec12; prolactin regulatory binding-element protein; Prolactin regulatory element-binding protein
Gene Aliases: C85705; PREB; SEC12
UniProt ID: (Human) Q9HCU5, (Mouse) Q9WUQ2
Entrez Gene ID: (Human) 10113, (Mouse) 50907
Molecular Function:
guanyl-nucleotide exchange factor
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