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PRELP Antibody (PA5-120649) in WB
Western blot analysis of PRELP in extracts of various cell lines. Samples were incubated with PRELP Polyclonal antibody (Product # PA5-120649) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-120649
Product Specifications
Species Reactivity
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 200-360 of human PRELP (NP_002716.1).
Conjugate
Form
Liquid
Concentration
3.14 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914221
Product Specific Information
Positive test controls include: Mouse lung, Rat heart.
Immunogen sequence: LDLQHNRLSD GVFKPDTFHG LKNLMQLNLA HNILRKMPPR VPTAIHQLYL DSNKIETIPN GYFKSFPNLA FIRLNYNKLT DRGLPKNSFN ISNLLVLHLS HNRISSVPAI NNRLEHLYLN NNSIEKINGT QICPNDLVAF HDFSSDLENV PHLRYLRLDG N
Target Information
PRELP is a leucine-rich repeat protein present in connective tissue extracellular matrix. This protein functions as a molecule anchoring basement membranes to the underlying connective tissue. This protein has been shown to bind type I collagen to basement membranes and type II collagen to cartilage. It also binds the basement membrane heparan sulfate proteoglycan perlecan. This protein is suggested to be involved in the pathogenesis of Hutchinson-Gilford progeria (HGP), which is reported to lack the binding of collagen in basement membranes and cartilage.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Prolargin; Proline-arginine-rich end leucine-rich repeat protein
Gene Aliases: 7330409J17Rik; Prelp; SLRR2A
UniProt ID: (Mouse) Q9JK53, (Rat) Q9EQP5
Entrez Gene ID: (Mouse) 116847, (Rat) 84400
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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