Western blot analysis of PREX1 using A) 30 µg 293T whole cell lysate and B) 30 µg whole cell lysate of human PREX1-transfected 293T cells (partial). Samples were loaded onto a 5% SDS-PAGE gel and probed with a PREX1 polyclonal antibody (Product # PA5-34960) at a dilution of 1:5000.
|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment contains a sequence corresponding to a region within amino acids 635 and 869 of PREX1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7, with 20% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Western Blot (WB)||1:1000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-34960 targets PREX1 in WB applications and shows reactivity with Human samples.
The PA5-34960 immunogen is recombinant fragment contains a sequence corresponding to a region within amino acids 635 and 869 of PREX1.
The protein encoded by this gene acts as a guanine nucleotide exchange factor for the RHO family of small GTP-binding proteins . It has been shown to bind to and activate RAC1 by exchanging bound GDP for free GTP. The encoded protein, which is found mainly in the cytoplasm, is activated by phosphatidylinositol-3,4,5-trisphosphate and the beta-gamma subunits of heterotrimeric G proteins.
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