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Invitrogen
PRIM1 Polyclonal Antibody
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FIGURE: 1 / 1
PRIM1 Antibody (PA5-116707) in WB
Western blot analysis of PRIM1 in extracts of Mouse spleen. Samples were incubated with PRIM1 Polyclonal antibody (Product # PA5-116707) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-116707
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 121-420 of human PRIM1 (NP_0009371)
Conjugate
Form
Liquid
Concentration
1.2 mg/mL
Amount
120 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Mouse spleen
Immunogen sequence: CCSSADICPK CWTLMTMAIR IIDRALKEDF GFKHRLWVYS GRRGVHCWVC DESVRKLSSA VRSGIVEYLS LVKGGQDVKK KVHLSEKIHP FIRKSINIIK KYFEEYALVN QDILENKESW DKILALVPET IHDELQQSFQ KSHNSLQRWE HLKKVASRYQ NNIKNDKYGP WLEWEIMLQY CFPRLDINVS KGINHLLKSP FSVHPKTGRI SVPIDLQKVD QFDPFTVPTI SFICRELDAI STNEEEKEEN EAESDVKHRT RDYKKTSLAP YVKVFEHFLE NLDKSRKGEL LKKSDLQKDF
Target Information
The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DNA primase 1; DNA primase 49 kDa subunit; DNA primase small subunit; DNA primase subunit 48; MGC12308; p49; PRI1; primase (DNA) subunit 1; primase p49 subunit; primase polypeptide 1, 49kDa; primase, DNA, polypeptide 1 (49kDa); unnamed protein product
Gene Aliases: AI324982; p49; PDIL; PRIM1
UniProt ID: (Human) P49642, (Mouse) P20664
Entrez Gene ID: (Human) 5557, (Mouse) 19075
magnesium ion binding
DNA-directed RNA polymerase activity
protein binding
zinc ion binding
transferase activity
nucleotidyltransferase activity
ribonucleotide binding
metal ion binding
telomerase activity
RNA helicase activity
DNA primase activity
sulfate adenylyltransferase activity
RNA guanylyltransferase activity
tRNA guanylyltransferase activity
mannose-phosphate guanylyltransferase activity
DNA/RNA helicase activity
CTP:2,3-di-O-geranylgeranyl-sn-glycero-1-phosphate cytidyltransferase activity
phospholactate guanylyltransferase activity
ATP:coenzyme F420 adenylyltransferase activity
UDP-N-acetylgalactosamine diphosphorylase activity
primase
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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