Proteintech

PRKACA Polyclonal Antibody

Product Image Gallery

PRKACA Antibody in Western Blot (WB) — Neuro-2a cells were subjected to SDS PAGE followed by western blot with 27398-1-AP (PRKACA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PRKACA Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human heart tissue slide using 27398-1-AP (PRKACA antibody) at dilution of 1:200 (under 10x lens).

PRKACA Antibody in Immunoprecipitation (IP) — IP result of anti-PRKACA (IP:27398-1-AP, 4ug; Detection:27398-1-AP 1:300) with HeLa cells lysate 2600 ug.

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2000

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

PRKACA Fusion Protein Ag26392

Conjugate

Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Target Information

Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitous signaling pathway important in the cell cycle, cellular communication, memory formation and behavior. PKA is composed of two catalytic (PKAc; Protein Kinase A catalytic subunit) and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimer and two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc can translocate into the nucleus and exert a regulatory role in the activation of multiple nuclear hormone receptors. However, PKAc-mediated activation of tonicity-dependent gene expression is cAMP independent. Humans express three types of PKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma are tissue-specific, the later is found in testes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C(s); C-alpha-2; C-alpha-S; cAMP-dependent protein kinase catalytic subunit alpha; cAMP-dependent protein kinase catalytic subunit alpha, isoform 1; MGC102831; MGC48865; PKA; PKA C-alpha; pkac; protein kinase A catalytic subunit; protein kinase, cAMP-dependent, alpha catalytic subunit; protein kinase, cAMP-dependent, catalytic, alpha

Gene Aliases: PKACA; PPNAD4; PRKACA

UniProt ID: (Human) P17612

Entrez Gene ID: (Human) 5566

Function(s)