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Invitrogen
PRKAR1A Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 8
PRKAR1A Antibody (PA5-79867) in ICC/IF
Immunofluorescence analysis of PRKAR1A was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with PRKAR1A Rabbit Polyclonal Antibody (Product # PA5-79867) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32731)... View More
Product Details
PA5-79867
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human PRKAR1A recombinant protein (Position: E2-E81).
Conjugate
Unconjugated
Form
Lyophilized
Concentration
500 µg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice
RRID
AB_2746982
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. This gene encodes one of the regulatory subunits. This protein was found to be a tissue-specific extinguisher that down-regulates the expression of seven liver genes in hepatoma x fibroblast hybrids. Mutations in this gene cause Carney complex (CNC). This gene can fuse to the RET protooncogene by gene rearrangement and form the thyroid tumor-specific chimeric oncogene known as PTC2. A nonconventional nuclear localization sequence (NLS) has been found for this protein which suggests a role in DNA replication via the protein serving as a nuclear transport protein for the second subunit of the Replication Factor C (RFC40). Three alternatively spliced transcript variants encoding the same protein have been observed.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cAMP-dependent protein kinase regulatory subunit RIalpha; cAMP-dependent protein kinase type I-alpha regulatory chain; cAMP-dependent protein kinase type I-alpha regulatory subunit; Carney complex type 1; protein kinase A type 1a regulatory subunit; protein kinase, cAMP dependent regulatory, type I, alpha; protein kinase, cAMP-dependent, regulatory subunit type I alpha; protein kinase, cAMP-dependent, regulatory, type I, alpha; Tissue-specific extinguisher 1; TSE1
Gene Aliases: ACRDYS1; ADOHR; CAR; CNC; CNC1; PKR1; PPNAD1; PRKAR1; PRKAR1A; RIIA; TSE1
UniProt ID: (Human) P10644, (Rat) P09456
Entrez Gene ID: (Human) 5573, (Rat) 25725
cAMP-dependent protein kinase inhibitor activity
protein binding
cAMP-dependent protein kinase regulator activity
cAMP binding
ubiquitin protein ligase binding
protein kinase A catalytic subunit binding
cytoskeletal protein
protein-binding activity modulator
kinase modulator
microtubule or microtubule-binding cytoskeletal protein
mesoderm formation
renal water homeostasis
regulation of transcription from RNA polymerase II promoter
female meiotic division
blood coagulation
activation of protein kinase A activity
intracellular signal transduction
sarcomere organization
negative regulation of meiotic nuclear division
negative regulation of activated T cell proliferation
cardiac muscle cell proliferation
cellular response to glucagon stimulus
negative regulation of cAMP-dependent protein kinase activity
negative regulation of protein kinase activity
heart development
regulation of protein kinase activity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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