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Invitrogen
PRKAR1B Polyclonal Antibody
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FIGURE: 1 / 29
PRKAR1B Antibody (PA5-101102) in IHC (P)
Immunohistochemistry analysis of PRKAR1B in human colorectal cancer and adjacent normal tissues. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. Samples were incubated with PRKAR1B polyclonal antibody (Product # PA5-101102) using a dilution of 1:100 (4°C overnight) followed by HRP conjugated anti-Rabbit secondary antibody.
Product Details
PA5-101102
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human PRKAR1B(Accession P31321), corresponding to amino acid residues I118-G168.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total KAP1.
Target Information
The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified that each represent specific gene products. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcrip- tion factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cAMP dependent protein kinase, subunit type 1 beta; cAMP dependent regulatory type I beta subunit transcript 2; cAMP dependent regulatory type I beta subunit transcript 3; cAMP dependent regulatory type I beta subunit transcript 4; cAMP-dependent protein kinase type I-beta regulatory subunit; protein kinase, cAMP-dependent, regulatory subunit type I beta; protein kinase, cAMP-dependent, regulatory, type I, beta; unnamed protein product
Gene Aliases: AI385716; MASNS; PRKAR1; PRKAR1B; RGD1563094; RIbeta
UniProt ID: (Human) P31321, (Rat) P81377, (Mouse) P12849
Entrez Gene ID: (Human) 5575, (Rat) 25521, (Mouse) 19085
adenylate cyclase-activating G-protein coupled receptor signaling pathway
learning or memory
modulation of synaptic transmission
regulation of synaptic vesicle cycle
negative regulation of cAMP/PKA signal transduction
positive regulation of long-term synaptic potentiation
positive regulation of fear response
positive regulation of excitatory postsynaptic potential
regulation of protein kinase activity
negative regulation of cAMP-dependent protein kinase activity
regulation of protein phosphorylation
protein phosphorylation
cell proliferation
organ morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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