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Invitrogen
PRKAR1B Polyclonal Antibody
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FIGURE: 1 / 2
PRKAR1B Antibody (PA5-87652) in WB
Western blot analysis of PRKAR1B in extracts of various cell lines. Samples were incubated with PRKAR1B Polyclonal antibody (Product # PA5-87652) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-87652
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 190-290 of human PRKAR1B (NP_0027261)
Conjugate
Form
Liquid
Concentration
1.3 mg/mL
Amount
130 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: WVTNISEGGS FGELALIYGT PRAATVKAKT DLKLWGIDRD SYRRILMGST LRKRKMYEEF LSKVSILESL EKWERLTVAD ALEPVQFEDG EKIVVQGEPG D; Positive Samples: U-251MG, HeLa, Jurkat, HepG2, BT-474, Mouse brain, Mouse lung, Rat brain, Rat testis; Cellular Location: Cell membrane
Target Information
The second messenger cyclic AMP (cAMP) mediates diverse cellular responses to external signals such as proliferation, ion transport, regulation of metabolism and gene transcription by activation of the cAMP-dependent protein kinase (cAPK or PKA). Activation of PKA occurs when cAMP binds to the two regulatory subunits of the tetrameric PKA holoenzyme resulting in release of active catalytic subunits. Three catalytic (C) subunits have been identified that each represent specific gene products. Activation of transcription upon elevation of cAMP levels results from translocation of PKA to the nucleus where it phosphorylates the transcrip- tion factor cAMP response element binding protein (CREB) on serine 133 which in turn leads to TFIIB binding to TATA-box-binding protein TBP1, thus linking phospho-CREB to the pol II transcription initiation complex.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cAMP dependent protein kinase, subunit type 1 beta; cAMP dependent regulatory type I beta subunit transcript 2; cAMP dependent regulatory type I beta subunit transcript 3; cAMP dependent regulatory type I beta subunit transcript 4; cAMP-dependent protein kinase type I-beta regulatory subunit; protein kinase, cAMP-dependent, regulatory subunit type I beta; protein kinase, cAMP-dependent, regulatory, type I, beta; unnamed protein product
Gene Aliases: AI385716; MASNS; PRKAR1; PRKAR1B; RGD1563094; RIbeta
UniProt ID: (Human) P31321, (Rat) P81377, (Mouse) P12849
Entrez Gene ID: (Human) 5575, (Rat) 25521, (Mouse) 19085
adenylate cyclase-activating G-protein coupled receptor signaling pathway
learning or memory
modulation of synaptic transmission
regulation of synaptic vesicle cycle
negative regulation of cAMP/PKA signal transduction
positive regulation of long-term synaptic potentiation
positive regulation of fear response
positive regulation of excitatory postsynaptic potential
regulation of protein kinase activity
negative regulation of cAMP-dependent protein kinase activity
regulation of protein phosphorylation
protein phosphorylation
cell proliferation
organ morphogenesis
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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