Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
10142-2-AP
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
PRKAR2A Fusion Protein Ag0203 (74-382 aa encoded by BC002763)
Conjugate
Form
Liquid
Concentration
0.31 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Wet ice (domestic); Dry ice (international)
Product Specific Information
This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55 kDa may be caused by phosphorylation.
Immunogen sequence: AKGDSESEE DEDLEVPVPS RFNRRVSVCA ETYNPDEEEE DTDPRVIHPK TDEQRCRLQE ACKDILLFKN LDQEQLSQVL DAMFERIVKA DEHVIDQGDD GDNFYVIERG TYDILVTKDN QTRSVGQYDN RGSFGELALM YNTPRAATIV ATSEGSLWGL DRVTFRRIIV KNNAKKRKMF ESFIESVPLL KSLEVSERMK IVDVIGEKIY KDGERIITQT KSNKDGGNQE VEIARCHKGQ YFGELALVTN KPRAASAYAV GDVKCLVMDV QAFERLLGPC MDIMKRNISH YEEQLVKMFG SSVDLGNLGQ (74-382 aa encoded by BC002763)
Target Information
cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of AMPK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cAMP-dependent protein kinase regulatory subunit RII alpha; cAMP-dependent protein kinase type II-alpha regulatory subunit; MGC3606; protein kinase A, RII-alpha subunit; protein kinase cAMP-dependent regulatory type II alpha; protein kinase, cAMP-dependent, regulatory subunit type II alpha; protein kinase, cAMP-dependent, regulatory, type 2, alpha
Gene Aliases: 1110061A24Rik; AI317181; AI836829; PKR2; PRKAR2; PRKAR2A; RII(alpha)
UniProt ID: (Human) P13861, (Rat) P12368
Entrez Gene ID: (Human) 5576, (Mouse) 19087, (Rat) 29699
cAMP-dependent protein kinase inhibitor activity
protein binding
cAMP-dependent protein kinase regulator activity
cAMP binding
ubiquitin protein ligase binding
protein kinase A catalytic subunit binding
nucleotide binding
protein domain specific binding
small molecule binding
protein homodimerization activity
kinase activity
protein-binding activity modulator
kinase modulator
renal water homeostasis
blood coagulation
activation of protein kinase A activity
intracellular signal transduction
cellular response to glucagon stimulus
negative regulation of cAMP-dependent protein kinase activity
regulation of protein phosphorylation
regulation of protein kinase activity
protein heterooligomerization
phosphorylation
response to clozapine
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support