Proteintech

PRKAR2A Polyclonal Antibody

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PRKAR2A Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells using 10142-2-AP (PRKAR2A antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

PRKAR2A Antibody in Western Blot (WB) — Human testis tissue were subjected to SDS PAGE followed by western blot with 10142-2-AP (PRKAR2A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

PRKAR2A Antibody in Immunoprecipitation (IP) — IP result of anti-PRKAR2A (IP:10142-2-AP, 4 ug; Detection:10142-2-AP 1:700) with K-562 cells lysate 2800 ug.

Product Details

10142-2-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:10-1:100	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PRKAR2A Fusion Protein Ag0203 (74-382 aa encoded by BC002763) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.31 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

This antibody got two bands about 45 kDa and 50-55 kDa in western blotting, and the 50-55 kDa may be caused by phosphorylation.

Immunogen sequence: AKGDSESEE DEDLEVPVPS RFNRRVSVCA ETYNPDEEEE DTDPRVIHPK TDEQRCRLQE ACKDILLFKN LDQEQLSQVL DAMFERIVKA DEHVIDQGDD GDNFYVIERG TYDILVTKDN QTRSVGQYDN RGSFGELALM YNTPRAATIV ATSEGSLWGL DRVTFRRIIV KNNAKKRKMF ESFIESVPLL KSLEVSERMK IVDVIGEKIY KDGERIITQT KSNKDGGNQE VEIARCHKGQ YFGELALVTN KPRAASAYAV GDVKCLVMDV QAFERLLGPC MDIMKRNISH YEEQLVKMFG SSVDLGNLGQ (74-382 aa encoded by BC002763)

Target Information

cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase (AMPK), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of AMPK is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of AMPK have been identified in humans. The protein encoded by this gene is one of the regulatory subunits. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of AMPK.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: cAMP-dependent protein kinase regulatory subunit RII alpha; cAMP-dependent protein kinase type II-alpha regulatory subunit; MGC3606; protein kinase A, RII-alpha subunit; protein kinase cAMP-dependent regulatory type II alpha; protein kinase, cAMP-dependent, regulatory subunit type II alpha; protein kinase, cAMP-dependent, regulatory, type 2, alpha

Gene Aliases: 1110061A24Rik; AI317181; AI836829; PKR2; PRKAR2; PRKAR2A; RII(alpha)

UniProt ID: (Human) P13861, (Rat) P12368

Entrez Gene ID: (Human) 5576, (Mouse) 19087, (Rat) 29699

Molecular Function: protein-binding activity modulator kinase modulator