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PRMT3 Polyclonal Antibody

View all (15) PRMT3 antibodies

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PRMT3 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 17628-1-AP (PRMT3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

PRMT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 17628-1-AP (PRMT3 antibody) at dilution of 1:100 (under 40x lens).

PRMT3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human brain using 17628-1-AP (PRMT3 antibody) at dilution of 1:100 (under 10x lens).

PRMT3 Antibody in Western Blot (WB) — Human liver tissue were subjected to SDS PAGE followed by western blot with 17628-1-AP (PRMT3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Product Details

17628-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:1,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PRMT3 Fusion Protein Ag11877 (178-531 aa encoded by BC037544) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.31 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: RAREDLQKM KQFAQDFVMH TDVRTCSSST SVIADLQEDE DGVYFSSYGH YGIHEEMLKD KIRTESYRDF IYQNPHIFKD KVVLDVGCGT GILSMFAAKA GAKKVLGVDQ SEILYQAMDI IRLNKLEDTI TLIKGKIEEV HLPVEKVDVI ISEWMGYFLL FESMLDSVLY AKNKYLAKGG SVYPDICTIS LVAVSDVNKH ADRIAFWDDV YGFKMSCMKK AVIPEAVVEV LDPKTLISEP CGIKHIDCHT TSISDLEFSS DFTLKITRTS MCTAIAGYFD IYFEKNCHNR VVFSTGPQST KTHWKQTVFL LEKPFSVKAG EALKGKVTVH KNKKDPRSLT VTLTLNNSTQ TYGLQ (178-531 aa encoded by BC037544)

Target Information

Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: heterogeneous nuclear ribonucleoprotein methyltransferase-like 3; Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3; HMT1 hnRNP methyltransferase-like 3; protein arginine methyltransferase 3; Protein arginine N-methyltransferase 3; protein arginine N-methyltransferase 3(hnRNP methyltransferase S. cerevisiae)-like 3

Gene Aliases: 2010005E20Rik; 2410018A17Rik; AL033309; HRMT1L3; PRMT3

UniProt ID: (Human) O60678, (Rat) O70467, (Mouse) Q922H1

Entrez Gene ID: (Human) 10196, (Rat) 89820, (Mouse) 71974

Molecular Function: protein modifying enzyme zinc finger transcription factor

regulation of transcription, DNA-templated peptidyl-arginine methylation, to asymmetrical-dimethyl arginine negative regulation of protein ubiquitination histone arginine methylation protein methylation peptidyl-arginine methylation dendritic spine morphogenesis methylation peptidyl-arginine N-methylation

Process(es)

protein binding methyltransferase activity histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity protein-arginine omega-N asymmetric methyltransferase activity metal ion binding S-adenosylmethionine-dependent methyltransferase activity modified amino acid binding rRNA (adenine-N6,N6-)-dimethyltransferase activity protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity transferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity