|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||synthetic peptide STKTHWKQTVFLLEKPF, corresponding to amino acids 456-472 of Human PRMT 3.|
|Contains||0.1% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|ChIP assay (ChIP)||10 µl for 10^6 cells|
|Immunohistochemistry (Paraffin) (IHC (P))||Assay-dependent|
|Western Blot (WB)||1:100-1:500|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-30273 detects PRMT3 from human samples.
PA1-30273 has been successfully used in Chimmunoprecipitation, ELISA, Western blot applications.
The PA1-30273 immunogen is: Synthetic peptide: STKTHWKQTVFLLEKPF, corresponding to amino acids 456-472 of Human PRMT 3.
Type I protein arginine N-methyltransferases (PRMTs), such as PRMT3, catalyze the formation of asymmetric N(G),N(G)-dimethylarginine (ADMA) residues in proteins (Tang et al., 1998 [PubMed 9642256]).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.