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Invitrogen
PRMT4 Polyclonal Antibody
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FIGURE: 1 / 4
PRMT4 Antibody (PA1-41103) in ICC/IF
Immunocytochemistry analysis of PRMT4 in HeLa cells. Samples were incubated with PRMT4 polyclonal antibody (Product # PA1-41103) using a dilution of 5 µg/mL overnight at 4°C followed by anti-rabbit Dylight 488 (Green) at a dilution of 1:500. Cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Product Details
PA1-41103
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mixture of synthetic peptides containing amino acids 45-69 and 595-608 of human PRMT4.
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Protein G
Storage buffer
PBS with 0.05% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive control: Hela whole cell extract, HeLa cell lysate.
Target Information
Protein arginine N-methyltransferases, such as CARM1, catalyze the transfer of a methyl group from S-adenosyl-L-methionine to the side chain nitrogens of arginine residues within proteins to form methylated arginine derivatives and S-adenosyl-L-homocysteine. Protein arginine methylation has been implicated in DNA packaging, transcription regulation, signal transduction, mRNA stability, metabolism of nascent pre-RNA, and transcriptional activation. Tissue specificity: Overexpressed in prostate adenocarcinomas and high-grade prostatic intraepithelial neoplasia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coactivator-associated arginine methyltransferase 1; Histone-arginine methyltransferase CARM1; Protein arginine N-methyltransferase 4
Gene Aliases: CARM1; PRMT4
UniProt ID: (Human) Q86X55
Entrez Gene ID: (Human) 10498
transcription coactivator activity
protein binding
beta-catenin binding
protein methyltransferase activity
histone-arginine N-methyltransferase activity
protein-arginine N-methyltransferase activity
ligand-dependent nuclear receptor transcription coactivator activity
protein-arginine omega-N asymmetric methyltransferase activity
histone methyltransferase activity (H3-R17 specific)
histone methyltransferase activity
protein homodimerization activity
transcription regulatory region DNA binding
lysine-acetylated histone binding
protein modifying enzyme
regulation of growth plate cartilage chondrocyte proliferation
transcription, DNA-templated
regulation of transcription, DNA-templated
DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest
aging
positive regulation of cell proliferation
viral process
histone methylation
peptidyl-arginine methylation, to asymmetrical-dimethyl arginine
intracellular estrogen receptor signaling pathway
negative regulation of protein binding
regulation of intracellular estrogen receptor signaling pathway
histone H3-R2 methylation
histone H3-R17 methylation
cellular lipid metabolic process
positive regulation of fat cell differentiation
response to cAMP
endochondral bone morphogenesis
regulation of mRNA binding
negative regulation of dendrite development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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