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          • Primary Antibodies ›
          • PRMT5 Antibodies

          Invitrogen

          PRMT5 Polyclonal Antibody

          View all (40) PRMT5 antibodies

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          Cite PRMT5 Polyclonal Antibody

          Additional Information:
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          • Antibody Testing Data (3)
          PRMT5 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          PRMT5 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          PRMT5 Antibody (PA1-41390) in WB

          Western blot was performed using PRMT5 Polyclonal Antibody (Product # PA1-41390) and bands of ~ 55, 66 kDa corresponding to PRMT5 was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), MCF-7 (Lane 2), T47D (Lane 3), Hep G2 (Lane 4) and NIH/3T3 (Lane 5) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0301BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          PRMT5 Antibody in Western Blot (WB)
          PRMT5 Antibody in Western Blot (WB)
          PRMT5 Antibody in Western Blot (WB)

          Product Details

          PA1-41390

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:1,000
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Mixture of synthetic peptides corresponding to human PRMT5 amino acid sequence.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Purification

          Protein G

          Storage buffer

          PBS with 100µg/mL BSA

          Contains

          0.02% sodium azide

          Storage conditions

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1086801

          Product Specific Information

          Suggested positive control: HeLa, NIH-3T3, Hela whole cell extract, antigen standard for PRMT5 (transient overexpression lysate).

          Target Information

          PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: 72 kDa ICln-binding protein; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; homolog of fission yeast Skb1; Jak-binding protein 1; JBP1; homodimer; PRMT5; Protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; SKB1; SKB1 homolog; unnamed protein product

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          Gene Aliases: HRMT1L5; HSL7; IBP72; JBP1; PRMT5; SKB1; SKB1Hs

          View more View less

          UniProt ID: (Human) O14744, (Mouse) Q8CIG8

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          Entrez Gene ID: (Human) 10419, (Mouse) 27374

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          Function(s)
          p53 binding transcription corepressor activity protein binding methyltransferase activity methyl-CpG binding histone-arginine N-methyltransferase activity protein-arginine N-methyltransferase activity transferase activity protein-arginine omega-N symmetric methyltransferase activity histone methyltransferase activity identical protein binding ribonucleoprotein complex binding histone methyltransferase activity (H4-R3 specific) macromolecular complex binding protein heterodimerization activity E-box binding histone H3 methyltransferase activity rRNA (adenine-N6,N6-)-dimethyltransferase activity core promoter sequence-specific DNA binding chromatin binding protein C-terminal carboxyl O-methyltransferase activity tRNA (guanine-N2-)-methyltransferase activity C-methyltransferase activity N-methyltransferase activity O-methyltransferase activity S-methyltransferase activity RNA methyltransferase activity mRNA methyltransferase activity tRNA methyltransferase activity protein methyltransferase activity 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity rRNA methyltransferase activity rRNA (uridine-2'-O-)-methyltransferase activity rRNA (adenine-N6-)-methyltransferase activity DNA-methyltransferase activity tRNA (guanine-N1-)-methyltransferase activity rRNA (cytosine-C5-)-methyltransferase activity selenocysteine methyltransferase activity arginine N-methyltransferase activity lysine N-methyltransferase activity protein-lysine N-methyltransferase activity tRNA (uracil) methyltransferase activity tRNA (guanine) methyltransferase activity tRNA (adenine) methyltransferase activity tRNA (cytosine) methyltransferase activity tRNA (cytosine-5-)-methyltransferase activity tRNA (adenine-N1-)-methyltransferase activity rRNA (adenine) methyltransferase activity rRNA (cytosine) methyltransferase activity rRNA (guanine) methyltransferase activity rRNA (uridine) methyltransferase activity protein C-terminal leucine carboxyl O-methyltransferase activity 1-phenanthrol methyltransferase activity protein-arginine N5-methyltransferase activity methylarsonite methyltransferase activity protein complex binding dimethylarsinite methyltransferase activity 4,5-dihydroxybenzo(a)pyrene methyltransferase activity 1-hydroxypyrene methyltransferase activity 1-hydroxy-6-methoxypyrene methyltransferase activity demethylmenaquinone methyltransferase activity cobalt-precorrin-6B C5-methyltransferase activity cobalt-precorrin-7 C15-methyltransferase activity cobalt-precorrin-5B C1-methyltransferase activity cobalt-precorrin-3 C17-methyltransferase activity dimethylamine methyltransferase activity hydroxyneurosporene-O-methyltransferase activity tRNA (adenine-57, 58-N(1)-) methyltransferase activity methylamine-specific methylcobalamin:coenzyme M methyltransferase activity trimethylamine methyltransferase activity methanol-specific methylcobalamin:coenzyme M methyltransferase activity monomethylamine methyltransferase activity P-methyltransferase activity Se-methyltransferase activity 2-phytyl-1,4-naphthoquinone methyltransferase activity tRNA (uracil-2'-O-)-methyltransferase activity tRNA (cytosine-2'-O-)-methyltransferase activity phosphomethylethanolamine N-methyltransferase activity tRNA (cytosine-3-)-methyltransferase activity rRNA (cytosine-2'-O-)-methyltransferase activity rRNA (cytosine-N4-)-methyltransferase activity trihydroxyferuloyl spermidine O-methyltransferase activity protein modifying enzyme
          Process(es)
          spliceosomal snRNP assembly chromatin organization chromatin remodeling DNA-templated transcription, termination regulation of transcription, DNA-templated regulation of mitotic nuclear division regulation of gene expression peptidyl-arginine methylation methylation circadian regulation of gene expression peptidyl-arginine N-methylation endothelial cell activation hypermethylation of CpG island negative regulation of cell differentiation negative regulation of transcription, DNA-templated positive regulation of mRNA splicing, via spliceosome rhythmic process positive regulation of oligodendrocyte differentiation regulation of ERK1 and ERK2 cascade Golgi ribbon formation liver regeneration regulation of signal transduction by p53 class mediator positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway negative regulation of transcription from RNA polymerase II promoter transcription, DNA-templated protein methylation chromatin modification peptidyl-arginine methylation, to symmetrical-dimethyl arginine histone arginine methylation histone H4-R3 methylation regulation of DNA methylation
          It has to be done as per old AB suggested Products section.

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