|Western Blot (WB)||1:500-1:1000|
|Tested Species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Human PRMT5 amino acid sequence.|
|Storage buffer||PBS with 100µg/ml BSA|
|Contains||0.02% sodium azide|
|Storage conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
Suggested positive control: HeLa, NIH-3T3, Hela whole cell extract, antigen standard for PRMT5 (transient overexpression lysate).
Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. PRMT5, also known as Janus kinase-binding protein 1 (JBP1) and Skb1Hs (human homolog of Skb1 of fission yeast can catalyze the formation of omega-N(G)-monomethylarginine and symmetric omega-N(G),N(G')-dimethylarginine in a variety of proteins. Recombinant PRMT5 can mono and dimethylate histone 2A and myelin basic protein. PRMT5 appears to have lower specific enzyme activity than PRMT1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 72 kDa ICln-binding protein; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; HRMT1L5; IBP72; Jak-binding protein 1; JBP1; Protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; SKB1; SKB1 homolog; SKB1Hs
Gene Aliases: HRMT1L5; IBP72; JBP1; PRMT5; SKB1; SKB1Hs
Molecular Function: signaling molecule
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