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FIGURE: 1 / 5
PRMT5 Antibody (712669) in ICC/IF
Knockdown of PRMT5 was achieved by transfecting MCF7 cells with specific siRNA (Silencer® select Product # s20377). Immunofluorescence analysis was performed on MCF7 cells (untransfected, panel a-d), transfected with PRMT5 specific siRNA (panel i-l) or non-specific scrambled siRNA (panels e-h). Cells were fixed, permeabilized, and labeled with Anti-PRMT5 Recombinant Rabbit Superclonal™ Antibody (Product # 712669, 1:100 dilution), followed by Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
Product Details
712669
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
Expi293
Class
Recombinant Superclonal
Type
Antibody
Clone
3HCLC
Immunogen
Protein corresponding to human PRMT5 [aa1-aa291]
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
Product Specific Information
This antibody is predicted to react with Monkey, Cat, Dog.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
Target Information
PRMT5 (protein arginine N-methyltransferase 5) is an arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. PRMT5 specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3; such methylation is required for the assembly and biogenesis of snRNP core particles.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 72 kDa ICln-binding protein; EC 2.1.1.125; Histone-arginine N-methyltransferase PRMT5; HMT1 hnRNP methyltransferase-like 5; homolog of fission yeast Skb1; Jak-binding protein 1; JBP1; homodimer; PRMT5; Protein arginine N-methyltransferase 5; Shk1 kinase-binding protein 1 homolog; SKB1; SKB1 homolog; unnamed protein product
Gene Aliases: HRMT1L5; HSL7; IBP72; JBP1; PRMT5; SKB1; SKB1Hs
UniProt ID: (Human) O14744
Entrez Gene ID: (Human) 10419
p53 binding
transcription corepressor activity
protein binding
methyltransferase activity
methyl-CpG binding
histone-arginine N-methyltransferase activity
protein-arginine N-methyltransferase activity
transferase activity
protein-arginine omega-N symmetric methyltransferase activity
histone methyltransferase activity
identical protein binding
ribonucleoprotein complex binding
histone methyltransferase activity (H4-R3 specific)
macromolecular complex binding
protein heterodimerization activity
E-box binding
histone H3 methyltransferase activity
spliceosomal snRNP assembly
chromatin organization
chromatin remodeling
DNA-templated transcription, termination
regulation of transcription, DNA-templated
regulation of mitotic nuclear division
regulation of gene expression
peptidyl-arginine methylation
methylation
circadian regulation of gene expression
peptidyl-arginine N-methylation
endothelial cell activation
hypermethylation of CpG island
negative regulation of cell differentiation
negative regulation of transcription, DNA-templated
positive regulation of mRNA splicing, via spliceosome
rhythmic process
positive regulation of oligodendrocyte differentiation
regulation of ERK1 and ERK2 cascade
Golgi ribbon formation
liver regeneration
regulation of signal transduction by p53 class mediator
positive regulation of adenylate cyclase-inhibiting dopamine receptor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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