Invitrogen

PRMT7 Polyclonal Antibody

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Datasheet

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Datasheet

Protocols

Questions & Answers

Tech Support

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PRMT7 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence was performed on fixed and permeabilized HeLa cells for detection of PRMT-7 using Anti- PRMT-7 Rabbit Polyclonal Antibody (Product # 720143, 1 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of PRMT-7 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating both nuclear and cytoplasmic localization of PRMT-7. Panel e) represents control cells with no primary Antibody to assess background.

PRMT7 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of A549 (Lane1), COS7 (Lane 2), HeLa (Lane 3), Jurkat (Lane 4) and HepG2 (Lane 5). The blots were probed with Anti- PRMT7 Rabbit Polyclonal Antibody (Product # 720143, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A clear 78 and 73 kDa band corresponding to PRMT7 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex®NuPAGE®4-12% Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Product Details

720143

Applications	Tested Dilution	Publications
Western Blot (WB)	1-2 µg/mL	-
Immunocytochemistry (ICC/IF)	1 µg/mL	-

Product Specifications
Species Reactivity	Human, Non-human primate
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human PRMT7 (aa 301-566) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.5 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS, pH 7.2, with 0.1% BSA, 30% glycerol
Contains	0.09% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2576972

Product Specific Information

These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature.

This antibody is predicted to react with Bovine, Rabbit and Goat.

Target Information

Arginine methylation is an irreversible post translational modification which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These proteins include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: [Myelin basic protein]-arginine N-methyltransferase PRMT7; FLJ10640; Histone-arginine N-methyltransferase PRMT7; myelin basic protein-arginine N-methyltransferase; PRMT-7; Protein arginine N-methyltransferase 7

Gene Aliases: KIAA1933; PRMT7

UniProt ID: (Human) Q9NVM4

Entrez Gene ID: (Human) 54496

Molecular Function: protein modifying enzyme

Function(s)

spliceosomal snRNP assembly regulation of gene expression by genetic imprinting transcription, DNA-templated regulation of transcription, DNA-templated histone methylation peptidyl-arginine methylation peptidyl-arginine methylation, to symmetrical-dimethyl arginine peptidyl-arginine methylation, to asymmetrical-dimethyl arginine cell differentiation histone arginine methylation DNA methylation involved in gamete generation regulation of protein binding histone H4-R3 methylation

Process(es)

histone-arginine N-methyltransferase activity S-adenosylmethionine-dependent methyltransferase activity protein-arginine N-methyltransferase activity [myelin basic protein]-arginine N-methyltransferase activity protein-arginine omega-N monomethyltransferase activity protein-arginine omega-N asymmetric methyltransferase activity protein-arginine omega-N symmetric methyltransferase activity histone binding ribonucleoprotein complex binding histone methyltransferase activity (H4-R3 specific)

PRMT7 Polyclonal Antibody

Product Details

720143

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Species Reactivity

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

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