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|Tested species reactivity||Human, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues 346-360 of human PRMT7.|
|Storage buffer||PBS with 0.05% BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Western Blot (WB)||1-3 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Suggested positive control: HeLa or NIH-3T3 whole cell lysate, antigen standard for PRMT7 (transient overexpression lysate).
Protein arginine methylation is an important posttranslational modification in eukaryotic cells, which has been implicated in RNA processing and trafficking, receptor-mediated signaling, and transcription. The PRMT proteins are recruited by C-terminal activation domain-2 of the SRC (steroid receptor coactivator) GRIP-1, whereas p300 interacts with activation domain-1 in the C terminus. have identified a mammalian arginine N-methyltransferase, PRMT7, that can catalyze the formation of omega-NG-monomethylarginine in peptides. In contrast to the other members of the PRMT family, PRMT7 was not able to methylate GST-fibrillarin or myelin basic protein or histone H2A, in vitro. It is also structurally different from other PRMTS in containing two putative AdoMet-binding motifs. Both of these domains are required for enzymatic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
[Myelin basic protein]-arginine N-methyltransferase PRMT7; FLJ10640; histone-arginine N-methyltransferase PRMT7; KIAA1933; myelin basic protein-arginine N-methyltransferase; protein arginine N-methyltransferase 7
4933402B05Rik; BC006705; KIAA1933; PRMT7