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Agrisera
PRP39a/pre-mRNA-processing factor 39 Polyclonal Antibody
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FIGURE: 1 / 1
PRP39a/pre-mRNA-processing factor 39 Antibody (AS14-2784) in WB
40 µg of total protein from 14-day-old seedlings of Arabidopsis thaliana was extracted with extraction buffer containing: 100 m M Tris HCl, 10% glycerol, 5 m M EGTA, 0.15 M Na Cl, 0.75% Triton X 100,0.05% SDS, 1 m M DTT, 1 x Complete Mini EDTA-free protease inhibitor (Roche) were separated on 14% SDS/PAGE using semi-dry transfer and blotted 1 h to PVDF. Blots were blocked with 5% milk in TBS+0.1% Tween ON a... View More
Product Details
AS14-2784
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant, full length PRP39a of Arabidopsis thaliana.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
PRP39a encodes a conserved U1 snRNP-associated splicing factor that localizes predominantly to the nucleus, where it contributes to early spliceosome assembly during pre-mRNA processing. The protein is built largely from repeated tetratricopeptide repeat (TPR)-like helical motifs that form an extended scaffold for protein-protein interactions and can assemble as a homodimer, a configuration proposed to functionally substitute for the Prp39/Prp42 heterodimer found in fungi. Functionally, PRP39a promotes efficient 5-prime splice-site recognition by facilitating U1 snRNP engagement, with experimental evidence indicating a particular impact on usage of weak 5-prime splice sites and broad effects on alternative splicing patterns.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT1G04080; F20D22.14; PRP39; PRP39-1
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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