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Invitrogen
PRPK Polyclonal Antibody
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FIGURE: 1 / 1
PRPK Antibody (PA5-90145) in WB
Western blot analysis of PRPK in extracts of various cell lines. Samples were incubated with PRPK Polyclonal antibody (Product # PA5-90145) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.
Product Details
PA5-90145
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-253 of human TP53RK (NP_2910283)
Conjugate
Form
Liquid
Concentration
1.37 mg/mL
Amount
137 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MAAARATTPA DGEEPAPEAE ALAAARERSS RFLSGLELVK QGAEARVFRG RFQGRAAVIK HRFPKGYRHP ALEARLGRRR TVQEARALLR CRRAGISAPV VFFVDYASNC LYMEEIEGSV TVRDYIQSTM ETEKTPQGLS NLAKTIGQVL ARMHDEDLIH GDLTTSNMLL KPPLEQLNIV LIDFGLSFIS ALPEDKGVDL YVLEKAFLST HPNTETVFEA FLKSYSTSSK KARPVLKKLD EVRLRGRKRS MVG; Positive Samples: HT-29, BxPC3, 293T, HeLa, Jurkat, Mouse testis, Mouse thymus, Rat testis; Cellular Location: Nucleus
Target Information
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine (PubMed:22912744, PubMed:27903914). The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37 (PubMed:22912744, PubMed:27903914). TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP. Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation (PubMed:11546806). [UniProt]
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Atypical serine/threonine protein kinase TP53RK; EKC/KEOPS complex subunit TP53RK; Nori-2; p53-related protein kinase; TP53 regulating kinase; TP53-regulating kinase; Trp53 regulating kinase; unnamed protein product
Gene Aliases: 2810408M09Rik; 4933401B08Rik; 5630401H01Rik; BUD32; C20orf64; dJ101A2; GAMOS4; mNori-2p; Nori-2; Nori-2p; PRPK; TP53RK; TPRKB; Trp53rk; Trp53tk
UniProt ID: (Human) Q96S44
Entrez Gene ID: (Human) 112858, (Rat) 362272, (Mouse) 76367
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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