Invitrogen

PRPS2 Polyclonal Antibody

3 Published Figures

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Questions & Answers

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Questions & Answers

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Antibody Testing Data (2)
Published Figures (3)

PRPS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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PRPS2 Antibody (PA5-42007) in IHC (P)

Immunohistochemistry (paraffin-embedded) analysis of human kidney tissue using an anti-PRPS2 polyclonal antibody (Product # PA5-42007).

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PRPS2 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry (paraffin-embedded) analysis of human kidney tissue using an anti-PRPS2 polyclonal antibody (Product # PA5-42007).

PRPS2 Antibody in Western Blot (WB) — Western blot analysis of human HepG2 cell lysate using an anti-PRPS2 polyclonal antibody (Product # PA5-42007).

PRPS2 Antibody in Immunocytochemistry (ICC/IF) — Figure 6 PRPS2 targetes E2F1 and activation of the P53/Bcl-xl/Bcl-2 signal pathway. ( a ) Colocation of PRPS2 and E2F1 in GC1 and GC2 cells and normal testis tissue was observed by double immunofluorescent staining. ( b ) After transfection, the expression levels of PRPS2 and E2F1 were detected by double immunofluorescent staining. ( c ) Western blot was used to measure the expression levels of the E2F1/P53/Bcl-xl/Bcl-2 signaling pathway. Values beneath the blots are relative to the control. White arrow: colocation. PRPS2: phosphoribosyl-pyrophosphate synthetase 2; NC: negative control; E2F1: E2F transcription factor 1.

PRPS2 Antibody in Western Blot, Immunohistochemistry (WB, IHC) — Figure 2 Correlation of PRPS2 expression with hypospermatogenesis. ( a ) H and E staining of the testis with normal spermatogenesis. ( b ) H and E staining of normal cauda epididymidis. ( c ) H and E staining of a testis with hypospermatogenesis. ( d ) H and E staining of cauda epididymidis in hypospermatogenesis. ( e ) PRPS2 expression was detected in normal testis by IHC. ( f ) PRPS2 expression was detected in a testis with hypospermatogenesis by IHC. Scale bars = 50 mum. ( g ) PRPS2 expression was detected in testis with normal spermatogenesis and hypospermatogenesis by Western blot. ( h ) PRPS2 expression was detected in testis tissues with normal spermatogenesis and hypospermatogenesis by qRT-PCR. *Hypospermatogenesis group compared with normal control, P < 0.05 by independent-samples t -test. Red arrow: spermatogonia; Black arrow: spermatocyte. PRPS2: phosphoribosyl-pyrophosphate synthetase 2; H and E: hematoxylinand and eosin; IHC: immunohistochemical; qRT-PCR: quantitative real-time polymerase chain reaction.

PRPS2 Antibody in Western Blot (WB) — Figure 1 PRPS2 depletion and apoptosis of spermatogenic cells. ( a ) qRT-PCR and Western blot analysis confirmed that PRPS2 was successfully downregulated by gene silencing and upregulated by PRPRS2 overexpression in both GC1 and GC2 cells. ( b ) Flow cytometry analysis shows that PRPS2 depletion promotes the apoptosis of GC1 cells. ( c ) Flow cytometry analysis shows that PRPS2 depletion promotes the apoptosis of GC2 cells (abscissa: cell count; ordinate: the fluorescence intensity). ( d ) Western blot analysis indicates that PRPS2 depletion activates the expression of apoptotic proteins (values beneath blots are relative to the control). *Significantly different compared with negative control, P < 0.05 by independent-samples t -test. shPRPS2: small-hairpin RNA gene silencer; NC: negative control; PRPS2: overexpression; PRPS2: phosphoribosyl-pyrophosphate synthetase 2; qRT-PCR: quantitative real-time polymerase chain reaction; APC-A: Allophycocyanin-A.

Product Details

PA5-42007

Applications

Tested Dilution

Publications

Western Blot (WB)

0.2-1 µg/mL

View 1 publication 1 publication

Immunohistochemistry (IHC)

-

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

4-8 µg/mL

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

-

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide directed towards the middle region of human PRPS2

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS with 2% sucrose

Contains

0.09% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2609479

Product Specific Information

Peptide sequence: VSPDAGGAKR VTSIADRLNV EFALIHKERK KANEVDRMVL VGDVKDRVAI

Sequence homology: Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%

Target Information

The function remains unknown.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

Published figure using PRPS2 polyclonal antibody (Product # PA5-42007) in Immunohistochemistry

Tue Aug 03 00:00:00 EDT 2021

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

Published figure using PRPS2 polyclonal antibody (Product # PA5-42007) in Immunohistochemistry

Tue Aug 03 00:00:00 EDT 2021

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

Published figure using PRPS2 polyclonal antibody (Product # PA5-42007) in Immunohistochemistry

Tue Aug 03 00:00:00 EDT 2021

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

Published figure using PRPS2 polyclonal antibody (Product # PA5-42007) in Immunohistochemistry

Tue Aug 03 00:00:00 EDT 2021

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

Published figure using PRPS2 polyclonal antibody (Product # PA5-42007) in Immunohistochemistry

Tue Aug 03 00:00:00 EDT 2021

Phosphoribosyl-pyrophosphate synthetase 2 (PRPS2) depletion regulates spermatogenic cell apoptosis and is correlated with hypospermatogenesis.

Asian journal of andrology

Lei B,Xie LX,Zhang SB,Wan B,Zhong LR,Zhou XM,Mao XM,Shu FP

Published figure using PRPS2 polyclonal antibody (Product # PA5-42007) in Immunohistochemistry

Tue Aug 03 00:00:00 EDT 2021

Bioinformatics

Protein Aliases: Phosphoribosyl pyrophosphate synthase II; PPRibP; PPRibP synthetase; PRS-II; ribose-phosphate diphosphokinase 2; Ribose-phosphate pyrophosphokinase 2; testicular secretory protein Li 41

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Gene Aliases: PRPS2; PRSII

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UniProt ID: (Human) P11908

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Entrez Gene ID: (Human) 5634

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Molecular Function: nucleotide kinase

5-phosphoribose 1-diphosphate biosynthetic process nucleobase-containing compound metabolic process nucleoside metabolic process ribonucleoside monophosphate biosynthetic process nucleotide biosynthetic process phosphorylation

Process(es)

magnesium ion binding ribose phosphate diphosphokinase activity protein binding ATP binding kinase activity identical protein binding protein homodimerization activity

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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