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Invitrogen
PRR5 Polyclonal Antibody
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FIGURE: 1 / 1
PRR5 Antibody (PA5-119923) in WB
Western blot analysis of PRR5 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. PRR5 Polyclonal Antibody (Product # PA5-119923) at 1:500 was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: MCF-7 cell lysate. Lane 2: Mouse liver tissue lysate. Lane 2: Rat liver tissue lysate.
Product Details
PA5-119923
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human PRR5 aa 200-388.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.4, with 50% glycerol, 0.2% BSA
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Control: MCF-7 cell lysate, mouse liver tissue lysate, rat liver tissue lysate.
Subcellular Location: Cytosol.
Target Information
MARK1 (MAP/microtubule affinity-regulating kinase 1) belongs to the MARK family of serine/threonine kinases. MARK family protein kinases phosphorylate microtubule-associated proteins and may play a role in cytoskeletal stability. MARK2 refers to MAP/microtubule affinity-regulating kinase 2 isoform a [Homo sapiens]. EMK (ELKL Motif Kinase) is a small family of ser/thr protein kinases involved in the control of cell polarity, microtubule stability and cancer. Several cDNA clones have been isolated that encoded two isoforms of the human ser/thr protein kinase EMK1 called MARK2. MARK3 was originally identified as a marker that was induced by treatment with DNA damaging agents, and loss of MARK3 was found with carcinogenesis in the pancreas. MARK4 contains an N terminal serine/threonine kinase domain, a central ubiquitin associated domain, and a C terminal KA1 associated kinase domain. RT PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: proline rich 5 (renal); Proline-rich protein 5; Protein observed with Rictor-1; Protor-1; prr5; prr5 {ECO:0000250|UniProtKB:P85299}; Rho GTPase activating protein 8; RP1-181C9.3
Gene Aliases: Arhgap8; AU043908; C030017C09Rik; C78947; FLJ20185k; PP610; PROTOR-1; PROTOR1; PRR5
UniProt ID: (Human) P85299, (Rat) Q5FVG6, (Mouse) Q812A5
Entrez Gene ID: (Human) 55615, (Rat) 315189, (Mouse) 109270
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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