A suggested positive control is NIH-3T3 cell lysate.
PA5-21129 can be used with blocking peptide PEP-1243.
Proline-rich protein 5-like (PRR5L), also known as Protor-2, is structurally similar to PRR5 and also binds to the mTOR complex 2 (mTORC2). PRR5L is not required for mTORC2 integrity or kinase activity, but dissociates from mTORC2 upon knockdown of tuberous sclerosis complex 1 (TSC1) and TSC2. Knockdown of PRR5L reduced apoptosis in mTORC2 deficient cells as well as cells where mTOR was active, suggesting that mTORC2-dissociated PRR5L may promote apoptosis, and thus play a role in the balance between cell growth and cell death.
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Protein Aliases: Proline-rich protein 5-like; Protein observed with Rictor-2; Protor-2
Gene Aliases: 2600010E01Rik; 4833411O04Rik; Protor-2; PROTOR2; PRR5L