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Invitrogen
PSAP Polyclonal Antibody
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FIGURE: 1 / 9
PSAP Antibody (PA5-21340) in ICC/IF
Immunofluorescence analysis of PSAP was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSAP Polyclonal Antibody (Product # PA5-21340) at 1:2000 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A270... View More
Product Details
PA5-21340
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human PSAP. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1.98 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 20% glycerol
Contains
0.025% ProClin 300
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: U87-MG, SK-N-SH, SK-N-AS, mouse brain, rat brain.
Predicted reactivity: Pig (87%), Bovine (86%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Proactivator polypeptide; Prosaposin; prosaposin (sulfated glycoprotein, sphingolipid hydrolase activator); SGP-1; snoRNA MBII-198; sphingolipid activator protein-1; Sulfated glycoprotein 1
Gene Aliases: AI037048; GLBA; PSAP; SAP1; SGP-1; Sgp1; SGP1A
UniProt ID: (Human) P07602, (Rat) P10960, (Mouse) Q61207
Entrez Gene ID: (Human) 5660, (Rat) 25524, (Mouse) 19156
platelet degranulation
glycosphingolipid metabolic process
lipid transport
adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway
regulation of autophagy
regulation of lipid metabolic process
positive regulation of catalytic activity
positive regulation of MAPK cascade
prostate gland growth
epithelial cell differentiation involved in prostate gland development
cellular response to organic substance
negative regulation of hydrogen peroxide-induced cell death
sphingolipid metabolic process
regulation of MAPK cascade
glycolipid transport
developmental growth
lipid metabolic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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