Invitrogen

PSAP Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

View all (23) PSAP antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

PSAP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PSAP was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSAP Polyclonal Antibody (Product # PA5-21340) at 1:2000 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing lysosomal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

PSAP Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft , using PSAP (Product # PA5-21340) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PSAP Antibody in Western Blot (WB) — Knockdown of PSAP was achieved by transfecting U-87 MG cells with PSAP specific siRNAs (Silencer® select Product # s11288, s11289). Western blot analysis (Fig. a) was performed using membrane enriched cell extracts from the PSAP knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with PSAP Polyclonal Antibody (Product # PA5-21340, 1:2000 dilution) Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PSAP.

PSAP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U-87 MG cells were transfected with PSAP siRNA and reduction of signal was observed in Western Blot using PSAP Polyclonal Antibody (Product # PA5-21340).

Product Details

PA5-21340

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:2,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1 and 250 of PSAP (Uniprot ID#P07602)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1.98 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11154619

Product Specific Information

Recommended positive controls: U87-MG, SK-N-SH, SK-N-AS, mouse brain, rat brain.

Predicted reactivity: Pig (87%), Bovine (86%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A1 activator; Cerebroside sulfate activator; Co-beta-glucosidase; Component C; CSAct; Dispersin; Glucosylceramidase activator; Proactivator polypeptide; Prosaposin; prosaposin (sulfated glycoprotein, sphingolipid hydrolase activator); Protein A; Protein C; SAP-1; SAP-2; Saposin-A; Saposin-B; Saposin-B-Val; Saposin-C; Saposin-D; SGP-1; snoRNA MBII-198; Sphingolipid activator protein 1; Sphingolipid activator protein 2; sphingolipid activator protein-1; Sulfated glycoprotein 1; Sulfatide/GM1 activator

Gene Aliases: AI037048; GLBA; PSAP; SAP1; SGP-1; Sgp1; SGP1A

UniProt ID: (Human) P07602, (Rat) P10960, (Mouse) Q61207

Entrez Gene ID: (Human) 5660, (Rat) 25524, (Mouse) 19156

Molecular Function: scaffold/adaptor protein