Invitrogen

PSAP Polyclonal Antibody

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

View all (22) PSAP antibodies

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Datasheet

Protocols

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PSAP Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of PSAP in U-2 OS cells using a PSAP polyclonal antibody (Product # PA5-82153). The analysis shows localization to vesicles.

PSAP Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of PSAP in human cerebral cortex and skeletal muscle tissues using a PSAP polyclonal antibody (Product # PA5-82153). Corresponding RNA-seq data are presented for the same tissues.

PSAP Antibody in Western Blot (WB) — Western blot analysis of PSAP by a PSAP polyclonal antibody (Product # PA5-82153). Analysis in human cell lines SK-MEL-30 and HEK293.

PSAP Antibody — Relative expression in different tissues in IHC: Detection of differential expression levels of PSAP demonstrates antibody specificity. Immunohistochemical analysis of PSAP using anti-PSAP Polyclonal Antibody (Product #PA5-82153), shows significant staining of PSAP in cerebral cortex and shows minimal or weak staining in skeletal muscle tissues. The relative expression levels of PSAP within each tissue is shown using RNA-Seq. {RE}

Product Details

PA5-82153

Applications	Tested Dilution	Publications
Western Blot (WB)	0.04-0.4 µg/mL	-
Immunohistochemistry (Paraffin) (IHC (P))	1:200-1:500	-
Immunocytochemistry (ICC/IF)	0.25-2 µg/mL	-

Product Specifications
Species Reactivity	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant protein corresponding to Human PSAP View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.1 mg/mL
Purification	Affinity Chromatography
Storage buffer	PBS, pH 7.2, with 40% glycerol
Contains	0.02% sodium azide
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2789314

Product Specific Information

Immunogen sequence: TNSTFVQALV EHVKEECDRL GPGMADICKN YISQYSEIAI QMMMHMQPKE ICALVGFCDE VKEMPMQTLV PAKVASKNVI PALELVEPIK KHEVPAKSDV YCEVCEFLVK EVTKLIDNNK TEKEILDAFD KMCSKLPKSL SEE

Target Information

PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: A1 activator; Cerebroside sulfate activator; Co-beta-glucosidase; Component C; CSAct; Dispersin; Glucosylceramidase activator; Proactivator polypeptide; Prosaposin; Protein A; Protein C; SAP-1; SAP-2; Saposin-A; Saposin-B; Saposin-B-Val; Saposin-C; Saposin-D; Sphingolipid activator protein 1; Sphingolipid activator protein 2; sphingolipid activator protein-1; Sulfatide/GM1 activator

Gene Aliases: GLBA; PSAP; SAP1

UniProt ID: (Human) P07602

Entrez Gene ID: (Human) 5660

Molecular Function: scaffold/adaptor protein