Invitrogen

PSAP Polyclonal Antibody

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PSAP Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of PSAP in U-2 OS cells using a PSAP polyclonal antibody (Product # PA5-82153). The analysis shows localization to vesicles.

PSAP Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of PSAP in human cerebral cortex and skeletal muscle tissues using a PSAP polyclonal antibody (Product # PA5-82153). Corresponding RNA-seq data are presented for the same tissues.

PSAP Antibody in Western Blot (WB) — Western blot analysis of PSAP by a PSAP polyclonal antibody (Product # PA5-82153). Analysis in human cell lines SK-MEL-30 and HEK293.

Product Details

Tested Applications

Dilution

Immunocytochemistry (ICC)

1-4 µg/mL

Immunofluorescence (IF)

1-4 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Western Blot (WB)

0.4 µg/mL

Product Specifications

Species Reactivity

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human PSAP

Conjugate

Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Product Specific Information

Immunogen sequence: TNSTFVQALV EHVKEECDRL GPGMADICKN YISQYSEIAI QMMMHMQPKE ICALVGFCDE VKEMPMQTLV PAKVASKNVI PALELVEPIK KHEVPAKSDV YCEVCEFLVK EVTKLIDNNK TEKEILDAFD KMCSKLPKSL SEE

Target Information

PSAP is a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: A1 activator; Cerebroside sulfate activator; Co-beta-glucosidase; Component C; CSAct; Dispersin; GLBA; Glucosylceramidase activator; Proactivator polypeptide; Prosaposin; Protein A; Protein C; SAP-1; SAP-2; Saposin-A; Saposin-B; Saposin-B-Val; Saposin-C; Saposin-D; Sphingolipid activator protein 1; Sphingolipid activator protein 2; sphingolipid activator protein-1; Sulfatide/GM1 activator

Gene Aliases: GLBA; PSAP; SAP1

UniProt ID: (Human) P07602

Entrez Gene ID: (Human) 5660

Function(s)

platelet degranulation glycosphingolipid metabolic process lipid transport adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway regulation of autophagy regulation of lipid metabolic process positive regulation of catalytic activity positive regulation of MAPK cascade prostate gland growth epithelial cell differentiation involved in prostate gland development cellular response to organic substance negative regulation of hydrogen peroxide-induced cell death

Process(es)

G-protein coupled receptor binding protein binding enzyme activator activity lipid binding

PSAP Polyclonal Antibody

Product Details

Product Specific Information

Target Information

Bioinformatics

Material safety data sheets (MSDS)

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