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Invitrogen
PSD-95 Polyclonal Antibody
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FIGURE: 1 / 2
PSD-95 Antibody (51-6900) in ICC/IF
Immunofluorescent analysis of PSD-95 (green) and MAP2 (red) on rat primary cortical neurons cultured for 28 days in the B-27 Plus Neuronal Culture System (Product # A3653401). At day 28 the cells were fixed with 4% paraformaldehyde for 15 min, permeabilized with 0.1% triton X-100 for 30min, and blocked with 1% BSA for 30 min at room temperature. Cells were stained with anti-PSD95 antibody (Product # 51-6900) at a dilution of 1:200, and anti-MAP2 (Product # 13-1500) at a dilution of 1:400, in 1% BSA staining buffer, overnight at 4C, and the... View More
Product Details
51-6900
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
C. elegans,
Chicken,
Human,
Mouse,
Rat,
Xenopus
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthethic peptide derived from the C-terminal region of human PSD-95.
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes the 95 kDa PSD-95 protein. Cross-reactivity with an unknown protein at ~50 kDa has been observed in western blots with rat brain homogenates. Western blots with mouse brain homogenates did not show such a band.
Positive reactivity of this antibody was confirmed on western blots of rat brain homogenates and lysates of PSD-95- transfected HEK293 (human embryonic kidney) cells.
Target Information
Post Synaptic Density 95 kDa (PSD-95), also known as synapse associated protein 90 kDa (SAP90), is one of a family of membrane-associated proteins found in the postsynaptic density in forebrain neurons and certain presynaptic structures in the cerebellum. Like other members of the family, PSD-95 has three 90 amino acid repeats called PDZ domains followed by an SH3 domain and a yeast guanylate kinase homology (GuK) domain. PSD-95 is believed to participate in the clustering of certain proteins, including NMDA receptors, Shaker-type potassium channels at the synaptic membrane in central nervous system (CNS) neurons. There are two principal modes of interaction between PSD-95 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif (beta 1 adrenergic receptor, some serotonin receptors, some sodium channel subunits, and additional potassium channel subunits), and some of these proteins may interact with PSD-95 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-95 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: discs large homolog 4; Disks large homolog 4; FLJ97752; FLJ98574; post synaptic density; post-synaptic density protein 95; Postsynaptic density protein 95; PSD-95; PSD-95 alpha 2b; PSD-95 beta; SAP-90; Synapse-associated protein 90; synapse-associated protein SAP90; Tax interaction protein 15
Gene Aliases: DLG4; Dlgh4; PSD-95; PSD95; SAP-90; SAP90; SAP90A
UniProt ID: (Human) P78352, (Rat) P31016, (Mouse) Q62108
Entrez Gene ID: (Human) 1742, (Rat) 29495, (Mouse) 13385
guanylate kinase activity
Ras guanyl-nucleotide exchange factor activity
protein binding
protein C-terminus binding
ligand-gated ion channel activity
kinase binding
protein phosphatase binding
PDZ domain binding
beta-1 adrenergic receptor binding
D1 dopamine receptor binding
P2Y1 nucleotide receptor binding
protein complex binding
acetylcholine receptor binding
ionotropic glutamate receptor binding
neuroligin family protein binding
scaffold protein binding
receptor binding
protein kinase binding
glutamate receptor binding
structural molecule activity
neurexin family protein binding
scaffold/adaptor protein
MAPK cascade
negative regulation of receptor internalization
protein complex assembly
signal transduction
positive regulation of cytosolic calcium ion concentration
chemical synaptic transmission
nervous system development
learning
synaptic vesicle maturation
ion transmembrane transport
social behavior
protein localization to synapse
locomotory exploration behavior
positive regulation of GTPase activity
establishment of protein localization
establishment or maintenance of epithelial cell apical/basal polarity
GMP metabolic process
GDP metabolic process
regulation of long-term neuronal synaptic plasticity
positive regulation of synaptic transmission
neuromuscular process controlling balance
dendritic spine morphogenesis
vocalization behavior
AMPA glutamate receptor clustering
receptor localization to synapse
regulation of N-methyl-D-aspartate selective glutamate receptor activity
positive regulation of excitatory postsynaptic potential
regulation of grooming behavior
neuronal ion channel clustering
nucleotide phosphorylation
locomotory behavior
response to cocaine
regulation of neuronal synaptic plasticity
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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