Invitrogen

PSD4 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

1 Reference

View all (4) PSD4 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Product Image Gallery

PSD4 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded human breast cancer, using PSD4 (Product # PA5-31837) antibody at 1:250 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

PSD4 Antibody in Western Blot (WB) — Western Blot using PSD4 Polyclonal Antibody (Product # PA5-31837). Sample (30 µg of whole cell lysate). Lane A: A431 . 7.5% SDS PAGE. PSD4 Polyclonal Antibody (Product # PA5-31837) diluted at 1:1,000.

PSD4 Antibody — Figure 3. KRAS* induces SDC1 membrane recycling through MAPK-PSD4-ARF6 axis. a-d , iKras p53 L/+ PDAC cells were grown in presence (ON) or absence (OFF) of doxycycline for 48 hours or indicated times. REON: cells were grown in absence of doxycycline for 48 hours followed by doxycycline treatment for 24 hours. Treatment of ON or REON samples with trametinib (50 nM), AZD8330 (100 nM), or BKM120 (100 nM) for 16-18 hours is indicated. ( a ) Surface Sdc1 was measured via FACS. ( b ) Cells were detached with trypsin to remove Sdc1 ectodomain and re-suspended in culture medium. (Top) Surface Sdc1 was labeled with anti-Sdc1 antibody at indicated times following re-suspension and measured via FACS. (Bottom) Mean Sdc1 levels. ( c ) Cells grown in presence (ON) or absence (OFF) of doxycycline for 48 hours were subjected to IF staining for Sdc1 (red), Arf6 (green) and DAPI (blue) (scale bar: 20 mum). ( d ) Cell lysates were immunoblotted for Psd4, phosphor-Akt, phosphor-Erk, Kras and ss-Actin. a-d: Data are representative of two independent experiments with similar results. {TM}

Product Details

PA5-31837

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	View 1 publication 1 publication
Immunohistochemistry (Paraffin) (IHC (P))	1:100-1:1,000	-

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Recombinant fragment corresponding to a region within amino acids 534 and 762 of Human PSD4 View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7, with 1% BSA, 20% glycerol
Contains	0.01% thimerosal
Storage conditions	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2549310

Product Specific Information

Recommended positive controls: A431.

Predicted reactivity: Mouse (89%), Rat (89%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Guanine nucleotide exchange factor for ARF6 and ARL14/ARF7. Through ARL14 activation, controls the movement of MHC class II-containing vesicles along the actin cytoskeleton in dendritic cells. Involved in membrane recycling. Interacts with several phosphatidylinositol phosphate species, including phosphatidylinositol 3,4-bisphosphate, phosphatidylinositol 3,5-bisphosphate and phosphatidylinositol 4,5-bisphosphate. [UniProt]

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Syndecan 1 is a critical mediator of macropinocytosis in pancreatic cancer.

Nature

Yao W,Rose JL,Wang W,Seth S,Jiang H,Taguchi A,Liu J,Yan L,Kapoor A,Hou P,Chen Z,Wang Q,Nezi L,Xu Z,Yao J,Hu B,Pettazzoni PF,Ho IL,Feng N,Ramamoorthy V,Jiang S,Deng P,Ma GJ,Den P,Tan Z,Zhang SX,Wang H,Wang YA,Deem AK,Fleming JB,Carugo A,Heffernan TP,Maitra A,Viale A,Ying H,Hanash S,DePinho RA,Draetta GF

PA5-31837 was used in Western Blotting to investigate if a mechanistic link between KRAS* signaling and a targetable molecule driving nutrient salvage pathway in pancreatic ductal adenocarcinoma exists.

Mon Apr 01 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: ADP-ribosylation factor guanine nucleotide-exchange factor 6; Exchange factor for ADP-ribosylation factor guanine nucleotide factor 6 B; exchange factor for ADP-ribosylation factor guanine nucleotide factor 6B; Exchange factor for ARF6 B; PH and SEC7 domain-containing protein 4; Pleckstrin homology and SEC7 domain-containing protein 4; SEC7 homolog; Telomeric of interleukin-1 cluster protein

Gene Aliases: EFA6B; PSD4; TIC

UniProt ID: (Human) Q8NDX1

Entrez Gene ID: (Human) 23550

Molecular Function: guanyl-nucleotide exchange factor

Function(s)

regulation of ARF protein signal transduction positive regulation of GTPase activity

Process(es)

ARF guanyl-nucleotide exchange factor activity phospholipid binding

PSD4 Polyclonal Antibody

Product Details

PA5-31837

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

RRID

Product Specific Information

Target Information

Bioinformatics

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