|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Rat, Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide corresponding to residues N(352) K L C D K P A S P R H Y S P(366) of human PSD-93.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS with 1mg/ml BSA|
|Contains||0.05% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||3-5 µg/10^6 cells|
|Immunohistochemistry (Frozen) (IHC (F))||5 µg/ml|
|Western Blot (WB)||3 - 5 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Western Blot (WB)||See 2 publications below|
PA1-043 detects Post Synaptic Density 93 (PSD-93) from rat and human tissues. This antibody does not detect other synapse-associated protein family members.
PA1-043 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects an ~110 kDa protein representing PSD-93 in rat brain extract. Immunohistochemical staining of PSD-93 in rat cerebellum with PA1-043 yields a pattern consistent with post-synaptic localization.
The PA1-043 immunogen is a synthetic peptide corresponding to residues N(352) K L C D K P A S P R H Y S P(366) of human PSD-93. This sequence is completely conserved in human PSD-93. PA1-043 immunizing peptide (Cat. # PEP-021) is available for use in neutralization and control experiments.
PSD-93, also known as chapsyn-110, is one of a family of plasma membrane-associated proteins found in synaptic junctions. PSD-93 is unique among family members in its expression in Purkinje neuron cell bodies and dendrites. PSD-93 has three ~90 amino acid repeats called PDZ domains, a single interior SH3 domain, and a carboxyl-terminal guanylate kinase homology (GuK) domain that is enzymatically inactive. It is hypothesized that PDZ-domain interactions play a role in receptor and channel clustering which contributes to neuronal plasticiyt. PSD-93 is believed to participate in the clustering of certain proteins, including NMDA receptors and shaker-type potassium channels at the synaptic membrane. There are two principal modes of interaction between PSD-93 and other proteins. NMDA receptors and shaker-type potassium channels both share C-terminal sequence homology consisting of a threonine/serine-X-valine-COOH (T/SXV) motif. Other neuronal proteins that share this motif may interact with PSD-93 by binding to its PDZ domains. Neuronal nitric oxide synthase (nNOS), which lacks the T/SXV motif but which has its own PDZ domain, has been shown to associate with PSD-93 in vitro through a pseudo-homotypic PDZ-PDZ interaction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Deletion of densin-180 results in abnormal behaviors associated with mental illness and reduces mGluR5 and DISC1 in the postsynaptic density fraction.
PA1-043 was used in western blot to investigate the role of densin-180 in the organization and dynamics of postsynaptic signaling complexes
|Carlisle HJ,Luong TN,Medina-Marino A,Schenker L,Khorosheva E,Indersmitten T,Gunapala KM,Steele AD,O'Dell TJ,Patterson PH,Kennedy MB||The Journal of neuroscience : the official journal of the Society for Neuroscience (31:16194)||2011|
Guanylyl cyclase/PSD-95 interaction: targeting of the nitric oxide-sensitive alpha2beta1 guanylyl cyclase to synaptic membranes.
PA1-043 was used in western blot to investigate the interaction between the alpha2beta1 guanylyl cyclase isoform and PSD-95 in rat brain.
|Russwurm M,Wittau N,Koesling D||The Journal of biological chemistry (276:44647)||2001|