Invitrogen

PSMA6 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

2 Published Figures

1 Reference

View all (38) PSMA6 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

PSMA6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 11

PSMA6 Antibody (PA5-22288) in IHC (P)

Proteasome 20S alpha 6 antibody detects Proteasome 20S alpha 6 protein at cytosol and nucleus on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. Proteasome 20S alpha 6 antibody (Product # PA5-22288) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Product Image Gallery

PSMA6 Antibody — Fig. 5. Altered neural protein expression after 48 h of inhibitor treatment. Alterations of protein levels after proteasome inhibition were detected by immunoblot analysis (A) and the signal intensities subsequently quantified (B). Cell lysates were prepared from primary cortical co-cultures after 48 h inhibitor treatment. Identical protein concentrations were loaded on gels and signals normalized to Coomassie stained sister gels. Signal intensities are expressed in relation to the control (100%). After 48 h, effects on protein expression after inhibitor treatment became even more obvious for NFkappaB and Caspase 3. The prolonged inhibitor incubation at a dose of 0.01 uM additionally effected massively decreased levels for CREB and pCREB after 48 h Bortezomib application compared to ONX-0914. Further, treatment with 0.01 and 0.1 uM Bortezomib over 48 h lead to decreased protein levels for beta-actin, AMPA, Synaptophysin 1 and GFAP in comparison to ONX-0914-treated cultures. Expression levels for alpha6 subunit and Iba1 were unaffected by inhibitor treatment over 48 h (5B). CREB activation indicated by phosphorylation was, compared to 24 h (4C), nearly unaffected after 48 h (5C). Presented data are mean +-SEM of 2-3 technical replicates each from 3 independent experiments, B: Student's t -test, *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001, C: one-sample t -test against hypothetical value 1, # : p < 0.05 {TM}

Product Details

PA5-22288

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment contain a sequence corresponding to a region within amino acids 57 and 246 of Proteasome 20S alpha 6

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

0.1M tris glycine, pH 7, with 20% glycerol

Contains

0.01% thimerosal

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_11157668

Product Specific Information

Recommended positive controls: 293T, A431, H1299, HeLa, mouse liver, rat liver.

Predicted reactivity: Mouse (100%), Rat (100%), Xenopus laevis (87%), Pig (98%), Chicken (96%), Sheep (99%), Rhesus Monkey (100%), Chimpanzee (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. A pseudogene has been identified on the Y chromosome.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Low Neurotoxicity of ONX-0914 Supports the Idea of Specific Immunoproteasome Inhibition as a Side-Effect-Limiting, Therapeutic Strategy.

European journal of microbiology & immunology

von Brzezinski L,Säring P,Landgraf P,Cammann C,Seifert U,Dieterich DC

Published figure using PSMA6 polyclonal antibody (Product # PA5-22288) in Western Blot

Fri Sep 01 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: 27 kDa prosomal protein; alpha-1; Macropain iota chain; macropain subunit iota; MGC22756; MGC2333; MGC23846; Multicatalytic endopeptidase complex iota chain; PROS 27; PROS-27; prosomal P27K protein; proteasome (prosome, macropain) subunit, alpha type, 6; proteasome alpha 6 subunit; Proteasome iota chain; Proteasome subunit alpha type-6; Proteasome subunit alpha-1; proteasome subunit iota; PSA6; PSMA 6; testicular secretory protein Li 44

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Gene Aliases: IOTA; p27K; PROS27; PSMA6

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UniProt ID: (Human) P60900, (Mouse) Q9QUM9, (Rat) P60901

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Entrez Gene ID: (Human) 5687, (Mouse) 26443, (Rat) 29673

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Function(s)

RNA binding endopeptidase activity threonine-type endopeptidase activity protein binding purine ribonucleoside triphosphate binding NF-kappaB binding peptidase activity hydrolase activity protease

Process(es)

MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability regulation of inflammatory response T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition proteolysis involved in cellular protein catabolic process Wnt signaling pathway, planar cell polarity pathway negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway proteolysis ubiquitin-dependent protein catabolic process skeletal muscle tissue development proteasomal ubiquitin-independent protein catabolic process

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PSMA6 Polyclonal Antibody

PSMA6 Antibody (PA5-22288) in IHC (P)

Product Details

PA5-22288

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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