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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • PSMB5 Antibodies

          Invitrogen

          PSMB5 Polyclonal Antibody

          Advanced Verification
          6 Published Figures
          14 References
          View all (11) PSMB5 antibodies

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          Datasheet
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          Datasheet
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          Cite PSMB5 Polyclonal Antibody

          • Antibody Testing Data (7)
          • Published Figures (6)
          • Advanced Verification (1)
          PSMB5 Antibody in Immunocytochemistry (ICC/IF)
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          PSMB5 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 14

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          PSMB5 Antibody (PA1-977) in ICC/IF

          Immunofluorescence analysis of PSMB5 was performed using 70% confluent log phase DU 145 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with PSMB5 Polyclonal Antibody (Product # PA1-977) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A327... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          PSMB5 Antibody in Immunocytochemistry (ICC/IF)
          PSMB5 Antibody in Immunocytochemistry (ICC/IF)
          PSMB5 Antibody in Immunocytochemistry (ICC/IF)
          PSMB5 Antibody in Immunocytochemistry (ICC/IF)
          PSMB5 Antibody in Western Blot (WB)
          PSMB5 Antibody in Western Blot (WB)
          PSMB5 Antibody in Western Blot (WB)
          PSMB5 Antibody in Immunocytochemistry (ICC/IF)
          PSMB5 Antibody in Western Blot (WB)
          PSMB5 Antibody in Western Blot (WB)
          PSMB5 Antibody in Western Blot (WB)
          PSMB5 Antibody in Western Blot (WB)
          PSMB5 Antibody in Western Blot (WB)
          PSMB5 Antibody

          Product Details

          PA1-977

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:5,000
          View 10 publications 10 publications

          Immunohistochemistry (PFA fixed) (IHC (PFA))

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:10-1:200
          View 3 publications 3 publications
          Product Specifications

          Species Reactivity

          Bovine, Human, Mouse

          Published species

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Synthetic Peptide: C W(243) I R V S S D N V A D L H D K Y S(259)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2172052

          Product Specific Information

          PA1-977 detects proteasome 20S X from bovine, mouse and human samples.

          PA1-977 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~23 kDa protein representing proteasome 20S X.

          The PA1-977 immunizing peptide corresponds to amino acid residues 243-259 from mouse proteasome 20S X. This sequence is 94% conserved in human and rat. PA1-977 immunizing peptide (Cat. # PEP-172) is available for use in neutralization and control procedures.

          Target Information

          Cyclic nucleotide phosphodiesterases.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: beta-5; Macropain epsilon chain; MGC118075; Multicatalytic endopeptidase complex epsilon chain; proteasome (prosome, macropain) subunit, beta type, 5; proteasome beta 5 subunit; proteasome beta type subunit 5; proteasome catalytic subunit 3; Proteasome chain 6; Proteasome epsilon chain; proteasome subunit beta 5; Proteasome subunit beta type-5; Proteasome subunit beta-5; Proteasome subunit MB1; Proteasome subunit X; proteasome subunit, beta type, 5; PSX large multifunctional protease X; testicular tissue protein Li 153

          View more View less

          Gene Aliases: LMPX; MB1; PSMB5; X

          View more View less

          UniProt ID: (Human) P28074, (Bovine) Q32KL2, (Mouse) O55234

          View more View less

          Entrez Gene ID: (Human) 5693, (Bovine) 534640, (Mouse) 19173

          View more View less

          Function(s)
          threonine-type endopeptidase activity protein binding peptidase activity endopeptidase activity hydrolase activity protease
          Process(es)
          MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent proteolysis regulation of cellular amino acid metabolic process response to oxidative stress viral process anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway proteasomal ubiquitin-independent protein catabolic process proteolysis involved in cellular protein catabolic process
          It has to be done as per old AB suggested Products section.
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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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