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Invitrogen
PSMB6 Polyclonal Antibody
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FIGURE: 1 / 9
PSMB6 Antibody (PA1-978) in ICC/IF
Immunofluorescent analysis of Proteasome 20S Y (green) in 3T3 cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes and blocked with 3% Blocker BSA (Product # 37525) in PBS for 30 minutes at room temperature. Cells were stained with or without Proteasome 20S Y rabbit polyclonal antibody (Product # PA1-978), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Goat anti-Rabbit (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor... View More
Product Details
PA1-978
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human,
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptides: C(41) R S G S A A D T Q A V/I A D A V T Y(58)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-978 detects proteasome 20S Y from purified bovine and human 26S proteasome samples.
PA1-978 has been successfully used in Western blot and ICC/IF procedures. By Western blot, this antibody detects an ~25 kDa protein representing proteasome 20S Y from purified human 26S proteasome.
The PA1-978 immunizing peptides correspond to amino acid residues 41-58 from human proteasome 20S Y and amino acid residues 74-91 from frog. These sequences are completely conserved in mouse, rat, and zebrafish. PA1-978 immunizing peptide (Cat. # PEP-173) is available for use in neutralization and control procedures.
Target Information
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signaling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Other proteins are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum. The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. The PA28/11S REG Activator protein complex functions as a proteolytic activator. This complex, consisting of alpha, beta, and gamma subunits, enhances the activity of the 20S proteolytic core. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis (CF transmembrane regulator), Angelmane's syndrome (E6-AP), and Liddle syndrome (endothelial sodium channels).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: beta-1; Low molecular mass protein 19; Macropain delta chain; Multicatalytic endopeptidase complex delta chain; proteasome (prosome, macropain) subunit, beta type, 6; proteasome beta 6 subunit; proteasome catalytic subunit 1; Proteasome chain 5; Proteasome delta chain; proteasome subunit beta 6; Proteasome subunit beta type-6; Proteasome subunit beta-1; proteasome subunit beta1; proteasome subunit delta; Proteasome subunit Y; PSMB6; PSY large multifunctional protease Y; RP23-122P1.4; subunit 2; unnamed protein product
Gene Aliases: DELTA; Lmp19; LMPY; Mpnd; PSMB6; Psmb6l; Y
UniProt ID: (Human) P28072, (Bovine) Q3MHN0, (Rat) P28073, (Mouse) Q60692
Entrez Gene ID: (Human) 5694, (Bovine) 510069, (Rat) 29666, (Mouse) 19175
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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