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Invitrogen
PSMB8 Polyclonal Antibody
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FIGURE: 1 / 5
PSMB8 Antibody (PA1-972) in ICC/IF
Immunofluorescent analysis of Proteasome 20S LMP7 (green) in HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) in PBS for 15 minutes at room temperature. Cells were stained with or without Proteasome 20S LMP7 rabbit polyclonal antibody (Product # PA1-972), at a concentration of 5 µg/mL for 1 hour at room temperature, and then incubated with a Alexa Fluor® 488 Superclonal goat anti-rabbit IgG secondary antibo... View More
Product Details
PA1-972
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C V(259) E S T D V S D L L H Q Y R E A(274)
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
PA1-972 detects Proteasome 20S LMP7 in western blot and immunofluorescence.
PA1-972 can be used with blocking peptide PEP-132.
Target Information
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DAAP-57C1.3; Low molecular mass protein 7; low molecular weight protein 7; Macropain subunit C13; MGC1491; Multicatalytic endopeptidase complex subunit C13; OTTHUMP00000029420; OTTHUMP00000029421; OTTHUMP00000062981; protease component C13; proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7); proteasome catalytic subunit 3i; Proteasome component C13; proteasome subunit beta 5i; Proteasome subunit beta type-8; Proteasome subunit beta-5i; proteasome subunit Y2; proteasome-related gene 7; Really interesting new gene 10 protein
Gene Aliases: ALDD; D6S216; D6S216E; JMP; LMP7; NKJO; PSMB5i; PSMB8; RING10; Y2
UniProt ID: (Human) P28062
Entrez Gene ID: (Human) 5696
MAPK cascade
protein polyubiquitination
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
regulation of cellular amino acid metabolic process
viral process
anaphase-promoting complex-dependent catabolic process
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of mRNA stability
fat cell differentiation
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
regulation of endopeptidase activity
Wnt signaling pathway, planar cell polarity pathway
type I interferon signaling pathway
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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