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PSMD14 Antibody (A303-857A) in WB
Detection of human and mouse PSMD14 by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg), HeLa (H; 50 µg), Jurkat (J; 50 µg) and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-PSMD14 antibody (Product # A303-857A) (lot A303-857A-1) used for WB at 0.4 µg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Product Details
A303-857A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 260 and 310 of Human Proteasome (Prosome, Macropain) 26S Subunit, non-ATPase, 14.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Wet ice
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
The 26S proteasome is responsible for most intracellular proteolysis in eukaryotes. POH1 (also known as Rpn11 in yeast) subunit of the 19S complex is responsible for substrate deubiquitination during proteasomal degradation. POH1 appears to be a Zn21-dependent protease, unlike all other known deubiquitinating enzymes (DUBs) that are cysteine proteases. POH1 was first identified as the human functional homolog of the S. pombe pad1 gene, which confers moderate resistance to chemotherapeutic drugs, and also to ultraviolet light when transiently overproduced in mammalian cells. Poh1-induced drug resistance is mediated through AP-1 transcription factors.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 26S proteasome non-ATPase regulatory subunit 14; 26S proteasome non-ATPase subunit; 26S proteasome regulatory subunit RPN11; 26S proteasome-associated pad1 homolog; 26S proteasome-associated PAD1 homolog 1; MAD1; proteasome (prosome, macropain) 26S subunit, non-ATPase, 14; proteasome 26S subunit, non-ATPase 14; testis tissue sperm-binding protein Li 69n
Gene Aliases: 2610312C03Rik; 3200001M20Rik; AA986732; PAD1; POH1; PSMD14; rpm11; RPN11
UniProt ID: (Human) O00487, (Mouse) O35593
Entrez Gene ID: (Human) 10213, (Mouse) 59029
MAPK cascade
protein polyubiquitination
double-strand break repair via homologous recombination
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
double-strand break repair via nonhomologous end joining
ubiquitin-dependent protein catabolic process
regulation of cellular amino acid metabolic process
positive regulation of endopeptidase activity
anaphase-promoting complex-dependent catabolic process
tumor necrosis factor-mediated signaling pathway
NIK/NF-kappaB signaling
Fc-epsilon receptor signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
regulation of mRNA stability
response to ethanol
T cell receptor signaling pathway
negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle
positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition
Wnt signaling pathway, planar cell polarity pathway
regulation of proteasomal protein catabolic process
protein K63-linked deubiquitination
negative regulation of canonical Wnt signaling pathway
positive regulation of canonical Wnt signaling pathway
DNA repair
proteolysis
cellular response to DNA damage stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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