Invitrogen

PSMD2 Polyclonal Antibody

1 Reference

View all (41) PSMD2 antibodies

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Datasheet

Protocols

Tech Support

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PSMD2 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of PSMD2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSMD2 Rabbit Polyclonal Antibody (Product # PA1-962) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the control without primary antibody. The images were captured at 60X magnification.

PSMD2 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF7 (Lane 1), PC-3 (Lane 2), Hep G2 (Lane 3), SK-OV-3 (Lane 4), HeLa (Lane 5), COS-7 (Lane 6), Jurkat (Lane 7), A-431 (Lane 8), tissue extracts of Mouse Testis (Lane 9) and Rat Testis (Lane 10). The blot was probed with Anti-PSMD2 Rabbit Polyclonal Antibody (Product # PA1-962, 1:1,000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 100 kDa band corresponding to PSMD2 was observed across the cell lines and tissues tested. In addition, 50 kDa band was observed across cell lines which may correspond to another subunit of Proteasome 19S Subunit S5b. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Product Details

PA1-962

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunocytochemistry (ICC/IF)	1:250	-
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Dog, Hamster, Human, Mouse, Non-human primate, Rat
Published species	Not Applicable
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic Peptide: C A(278) T N R P D T L D P A L L R P G R(294)
Conjugate	Unconjugated
Form	Liquid
Concentration	Conc. Not Determined
Storage conditions	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2172782

Product Specific Information

PA1-962 detects proteasome 19S subunit 10B from canine, hamster, human, mouse and rat tissues and cells.

PA1-962 has been successfully used in Western blot procedures. By Western blot, this antibody detects ~42, 46, 48, and 52 kDa proteins which correspond to subunit 10B and probably the combined signal from 4, 6B, 7, and 8 subunit proteins.

PA1-962 immunizing peptide corresponds to amino acid residues 278-294 of human proteasome 19S subunit 10B. This sequence is conserved in 19S protein subunits S4 (~53 kDa), S6A (~53 kDa), S6B (~46 kDa), S7 (~47 kDa), and S8 (~45 kDa). PA1-962 immunizing peptide (Cat. # PEP-107) is available for use in neutralization and control experiments.

Target Information

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

The cyclophilin-like domain of Ran-binding protein-2 modulates selectively the activity of the ubiquitin-proteasome system and protein biogenesis.

The Journal of biological chemistry

Yi H,Friedman JL,Ferreira PA

PA1-962 was used in western blot to investigate the role of proteasome subunits S10B during the proteolysis of intracellular proteins regulated by the ubiquitin-proteasome system.

Fri Nov 30 00:00:00 EST 2007

Bioinformatics

Protein Aliases: 26S proteasome non-ATPase regulatory subunit 2; 26S proteasome non-ATPase regulatory subunit 2-like protein; 26S proteasome regulatory subunit RPN1; 26S proteasome regulatory subunit S2; 26S proteasome subunit p97; 55.11 protein; F23F1.8; MGC14274; proteasome (prosome, macropain) 26S subunit, non-ATPase, 2; Protein 55.11; testis expressed gene 190; TNFR-associated protein 2; Tumor necrosis factor type 1 receptor-associated protein 2

Gene Aliases: PSMD2; TRAP2