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Invitrogen
PSMD2 Polyclonal Antibody
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FIGURE: 1 / 3
PSMD2 Antibody (PA1-962) in ICC/IF
Immunofluorescence analysis of PSMD2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSMD2 Rabbit Polyclonal Antibody (Product # PA1-962) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More
Product Details
PA1-962
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic Peptide: C A(278) T N R P D T L D P A L L R P G R(294)
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
Product Specific Information
PA1-962 detects proteasome 19S subunit 10B from canine, hamster, human, mouse and rat tissues and cells.
PA1-962 has been successfully used in Western blot procedures. By Western blot, this antibody detects ~42, 46, 48, and 52 kDa proteins which correspond to subunit 10B and probably the combined signal from 4, 6B, 7, and 8 subunit proteins.
PA1-962 immunizing peptide corresponds to amino acid residues 278-294 of human proteasome 19S subunit 10B. This sequence is conserved in 19S protein subunits S4 (~53 kDa), S6A (~53 kDa), S6B (~46 kDa), S7 (~47 kDa), and S8 (~45 kDa). PA1-962 immunizing peptide (Cat. # PEP-107) is available for use in neutralization and control experiments.
Target Information
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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