Invitrogen

PSMD2 Polyclonal Antibody

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Antibody Testing Data (3)
Published Figures (2)

PSMD2 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 5

PSMD2 Antibody (PA1-964) in ICC/IF

Immunofluorescence analysis of PSMD2 was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with PSMD2 Rabbit Polyclonal Antibody (Product # PA1-964) at 1:250 dilution in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pro... View More

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Product Details

PA1-964

Applications

Tested Dilution

Publications

Western Blot (WB)

1:250

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:250

Immunoprecipitation (IP)

Assay-dependent

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Human recombinant proteasome 19S subunit S2.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_325947

Product Specific Information

PA1-964 detects proteasome 19S subunit S2 from human cells.

PA1-964 has been successfully used in Western blot and immunoprecipitation procedures. By Western blot, this antibody detects a 100 kDa protein representing proteasome 19S subunit S2 in HeLa cell lysate. This antibody detects, to a lesser extent an ~50-kDa protein which could correspond to subunit degradation product.

PA1-964 antigen is recombinant human proteasome 19S subunit S2.

Target Information

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid. In addition to participation in proteasome function, this subunit may also participate in the TNF signalling pathway since it interacts with the tumor necrosis factor type 1 receptor. A pseudogene has been identified on chromosome 1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

COP I and II dependent trafficking controls ER-associated degradation in mammalian cells.

iScience

Ogen-Shtern N,Chang C,Saad H,Mazkereth N,Patel C,Shenkman M,Lederkremer GZ

PA1-964 was used in Western Blot to suggest that targeting of misfolded proteins to ERAD involves COPII-dependent transport to the ERQC and that they can be retrieved to the peripheral ER in a COPI-dependent manner.

Fri Mar 17 00:00:00 EDT 2023

The Proteasome Ubiquitin Receptor hRpn13 and Its Interacting Deubiquitinating Enzyme Uch37 Are Required for Proper Cell Cycle Progression.

The Journal of biological chemistry

Randles L,Anchoori RK,Roden RB,Walters KJ

PA1-964 was used in western blot to elucidate the roles of hRpn13 and Uch37 in cell cycle progression

Fri Apr 15 00:00:00 EDT 2016

The capture proteasome assay: A method to measure proteasome activity in vitro.

Analytical biochemistry

Vigneron N,Abi Habib J,Van den Eynde BJ

PA1-964 was used in immunoprecipitation to develop the capture proteasome assay to assess proteasome activity in vitro using cell lysates.

Sat Aug 01 00:00:00 EDT 2015

Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.

Nature

Sarraf SA,Raman M,Guarani-Pereira V,Sowa ME,Huttlin EL,Gygi SP,Harper JW

PA1-964 was used in western blot to use a proteomics approch to identify the range of PARKIN substrates during mitochondrial depolarization

Thu Apr 18 00:00:00 EDT 2013

Proteasomal non-catalytic subunit PSMD2 as a potential therapeutic target in association with various clinicopathologic features in lung adenocarcinomas.

Molecular carcinogenesis

Matsuyama Y,Suzuki M,Arima C,Huang QM,Tomida S,Takeuchi T,Sugiyama R,Itoh Y,Yatabe Y,Goto H,Takahashi T

Published figure using PSMD2 polyclonal antibody (Product # PA1-964) in Western Blot

Fri Apr 01 00:00:00 EDT 2011

Proteasomal non-catalytic subunit PSMD2 as a potential therapeutic target in association with various clinicopathologic features in lung adenocarcinomas.

Molecular carcinogenesis

Matsuyama Y,Suzuki M,Arima C,Huang QM,Tomida S,Takeuchi T,Sugiyama R,Itoh Y,Yatabe Y,Goto H,Takahashi T

Published figure using PSMD2 polyclonal antibody (Product # PA1-964) in Western Blot

Fri Apr 01 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: 26S proteasome non-ATPase regulatory subunit 2; 26S proteasome regulatory subunit RPN1; 26S proteasome regulatory subunit S2; 26S proteasome subunit p97; 55.11 protein; F23F1.8; MGC14274; proteasome (prosome, macropain) 26S subunit, non-ATPase, 2; Protein 55.11; TNFR-associated protein 2; Tumor necrosis factor type 1 receptor-associated protein 2

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Gene Aliases: P97; PSMD2; RPN1; S2; TRAP2

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UniProt ID: (Human) Q13200

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Entrez Gene ID: (Human) 5708

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Function(s)

endopeptidase activity protein binding enzyme regulator activity protease

Process(es)

MAPK cascade protein polyubiquitination stimulatory C-type lectin receptor signaling pathway antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent regulation of cellular amino acid metabolic process anaphase-promoting complex-dependent catabolic process tumor necrosis factor-mediated signaling pathway NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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PSMD2 Polyclonal Antibody

PSMD2 Antibody (PA1-964) in ICC/IF

Product Details

PA1-964

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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