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PA1-974 detects proteasome 19S subunit S3 from human and mouse cells.
PA1-974 has been successfully used in ICC/IF, FACS, and Western blot procedures. By Western blot, this antibody detects an ~60 kDa protein representing proteasome 19S subunit S3 from HeLa cell extract.
For Western blot procedures it is necessary to transfer the protein to membrane at pH 11.
PA1-974 immunizing peptide corresponds to amino acid residues 513-534 from human proteasome 19S subunit S3 protein.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes one of the non-ATPase subunits of the 19S regulator lid.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 26S proteasome non-ATPase regulatory subunit 3; 26S proteasome regulatory subunit RPN3; 26S proteasome regulatory subunit S3; proteasome (prosome, macropain) 26S subunit, non-ATPase, 3; Proteasome subunit p58; tissue specific transplantation antigen 2; tissue specific transplantation antigen P91A; Transplantation antigen P91A; Tum-P91A antigen
Gene Aliases: AI255837; AntP91a; D11Bwg1349e; P58; P91a; Psd3; PSMD3; RPN3; S3; TSTA2; Tstap91a
UniProt ID: (Human) O43242, (Mouse) P14685
Entrez Gene ID: (Human) 5709, (Mouse) 22123
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