This Antibody was verified by IP Mass Spectrometry to ensure that the antibody binds to the antigen stated. View Details
PA1-960 detects proteasome activator 11S REG alpha subunit from canine, hamster, human, mouse and rat tissues and cells.
PA1-960 has been successfully used in Western blot procedures. By Western blot, this antibody detects a 28 kDa protein representing 11S REG alpha subunit in rat brain extract.
PA1-960 immunizing peptide corresponds to amino acid residues 5-22 of human proteasome activator 11S REG alpha. This sequence differs from the mouse and rat 11S REG alpha protein by a single amino acid residue. PA1-960 immunizing peptide (Cat. # PEP-097) is available for use in neutralization and control experiments.
Proteolytic degradation is critical to the maintenance of appropriate levels of short-lived and regulatory proteins as important and diverse as those involved in cellular metabolism, heat shock and stress response, antigen presentation, modulation of cell surface receptors and ion channels, cell cycle regulation, transcription, and signalling factors. The ubiquitin-proteasome pathway deconstructs most proteins in the eukaryotic cell cytosol and nucleus. Others are degraded via the vacuolar pathway which includes endosomes, lysosomes, and the endoplasmic reticulum.
The 26S proteasome is an ATP-dependent, multisubunit (~31), barrel-shaped molecular machine with an apparent molecular weight of ~2.5 MDa. It consists of a 20S proteolytic core complex which is crowned at one or both ends by 19S regulatory subunit complexes. The 19S regulatory subunits recognize ubiquitinated proteins and play an essential role in unfolding and translocating targets into the lumen of the 20S subunit. An enzymatic cascade is responsible for the attachment of multiple ubiquitin molecules to lysine residues of proteins targeted for degradation. Several genetic diseases are associated with defects in the ubiquitin-proteasome pathway. Some examples of affected proteins include those linked to cystic fibrosis, Angelman's syndrome, and Liddle syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: 11S regulator complex subunit alpha; 29-kD MCP activator subunit; Activator of multicatalytic protease subunit 1; epididymis secretory sperm binding protein Li 129m; IFI5111; IGUP I-5111; Interferon gamma up-regulated I-5111 protein; interferon-gamma IEF SSP 5111; interferon-gamma-inducible protein 5111; MGC8628; PA28 alpha subunit; PA28A; PA28alpha; protease (prosome, macropain) 28 subunit, alpha; proteasome (prosome, macropain) 28 subunit, alpha; proteasome (prosome, macropain) activator subunit 1 (PA28 alpha); Proteasome activator 28 subunit alpha; Proteasome activator complex subunit 1; REG-alpha; REGalpha
Gene Aliases: AW413925; HEL-S-129m; I79_008549; IFI5111; PA28A; PA28alpha; PSME1; REGalpha